重组小鼠BMP-4蛋白（Recombinant Murine BMP-4 Protein）

货 号：R-TL680

产品描述（Description）

BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. Mature mouse and human BMP-4 share 98% aa sequence identity. Mouse BMP-4 shares 85% aa sequence identity with mouse BMP-2 and 35%-54% with other mouse BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation. In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.

同义词（Alternative Names）

Bmp-4 Protein,Mouse、Bmp2b Protein,Mouse、Bmp2b-1 Protein,Mouse、Bmp2b1 Protein

蛋白序列（Protein Sequence）

DNA 序列编码小鼠 BMP-4（ NCBI Reference Sequence: NP_031580.2）表达带有 Fc 标签在 C 末端

分子量（Molecular Weight）

重组小鼠 BMP-4蛋白 包含 339 个氨基酸，预测理论分子量为 38.9 kD

纯度（Purity）

>90%，采用 SDS-PAGE 凝胶分析

内毒素（Endotoxin）

<1EU/μg

表达宿主（Expression host）

HEK293 细胞

生物活性（Biological Activity）

通过 ATDC-5 细胞诱导碱性磷酸酶的产生来确定其活性。预期的 ED₅₀为 10-30ng/mL

纯化方式（ Purification Method ）

层析纯化

性状（Character）

白色疏松体

使用说明（Instructions）

冻干制剂可在 2~8℃保存 24 个月。 

如需分装，可用注射用水、生理盐水、培养基或 PBS 溶解，溶解后分装成小份，置于-20℃保存 期 6 个月，-80℃保存期 12 个月，稀释后浓度不低于 100μg/mL。避免反复冻融。

  注意：For research and manufacturing use.