整合素αX抗体-抗体-抗体-生物在线
上海雅吉生物科技有限公司
整合素αX抗体

整合素αX抗体

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产品名称： 整合素αX抗体

英文名称： beta Glucan Receptor

产品编号： 2445

产品价格： null

产品产地： 上海

品牌商标： 雅吉

更新时间： null

使用范围： ELISA Flow-Cyt IF

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中文名称 整合素αX抗体
别    名 Integrin alpha-X; integrin alpha-X precursor; ITGAX; CD11C; SLEB6; CD11C (p150) alpha polypeptide; complement receptor 4; integrin aX; CD11 antigen-like family member C; leukocyte adhesion receptor p150,95; leukocyte adhesion glycoprotein p150,95 alpha chain; ITAX_HUMAN. 

 

研究领域 细胞生物  免疫学  信号转导  干细胞  细胞粘附分子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat,  (predicted: Mouse, Dog, )
产品应用 ELISA=1:500-1000 Flow-Cyt=1μg/Test IF=1:200-800 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 126kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Integrin Alpha X:501-600/1163 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. [provided by RefSeq].

Function:
Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.

Subunit:
Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain.

Subcellular Location:
Membrane.

Tissue Specificity:
Predominantly expressed in monocytes and granulocytes.

Similarity:
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.

SWISS:
P20702

Gene ID:
3687

Database links:

Entrez Gene: 3687 Human

Entrez Gene: 16411 Mouse

Entrez Gene: 499271 Rat

Omim: 151510 Human

SwissProt: P20702 Human

SwissProt: Q9QXH4 Mouse

Unigene: 248472 Human

Unigene: 22378 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD11c) Polyclonal Antibody, Unconjugated (bs-2508R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-CD11c antibody (bs-2508R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-CD11c antibody (bs-2508R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control (blue): U937 cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-CD11c antibody(bs-2508R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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