Anti-Polyethylene glycol抗体[RM105]-抗体-抗体-生物在线

产品名称： Anti-Polyethylene glycol抗体[RM105]

英文名称： PEG antibody

产品编号： ab190652

产品价格： null

产品产地：英国

品牌商标： abcam

更新时间： null

使用范围：

深圳市宇德立生物科技有限公司

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经测试应用ICC, WB, ELISA, IHC-P, Flow Cyt, IPmore details
免疫原
Chemical/ Small Molecule corresponding to Polyethylene glycol conjugated to Keyhole Limpet Haemocyanin (KLH). (KLH-PEG with terminal methoxy group).
阳性对照
- Mouse liver and kidney tissue injected with PEG-BSA; PEGylated BSA.
常规说明
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents. Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents
when performing ELISA using anti-PEG products.

Learn more about PEG RabMAb products and kit - PEG RabMAb Product Portal Page

形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 98% PBS
浓度100 µl 浓度为 1 mg/ml
纯度Protein A purified
纯化说明ab190652 was purified from an animal origin–free and protein-free culture supernatant.
克隆单克隆
克隆编号RM105
同种型IgG
研究领域

Our Abpromise guarantee covers the use of ab190652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Abreviews	说明
ICC		Use at an assay dependent concentration.
WB		Use a concentration of 0.05 - 1 µg/ml.
ELISA		Use a concentration of 0.01 - 0.3 mg/ml.
IHC-P		Use a concentration of 0.5 - 2 µg/ml.
Flow Cyt		Use at an assay dependent concentration. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP		Use at an assay dependent concentration.

Western blot - Anti-Polyethylene glycol antibody [RM105] (ab190652)

All lanes : Anti-Polyethylene glycol antibody [RM105] (ab190652) at 0.1 µg/ml

Lane 1 : BSA at 0.2 µg
Lane 2 : PEGylated BSA at 0.2 µg
Lane 3 : BSA at 0.1 µg
Lane 4 : PEGylated BSA at 0.1 µg
Lane 5 : BSA at 0.05 µg
Lane 6 : PEGylated BSA at 0.05 µg
Lane 7 : BSA at 0.02 µg
Lane 8 : PEGylated BSA at 0.02 µg
Lane 9 : BSA at 0.01 µg
Lane 10 : PEGylated BSA at 0.01 µg
ELISA - Anti-Polyethylene glycol antibody [RM105] (ab190652)

ELISA of three different PEGylated BSA using ab190652 at 0.3 µg/ml, following AP conjugated goat anti-rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [RM105] (ab190652)

Immunohistochemical analysis of Mouse liver and kidney tissue labeling PEG with ab190652 at 0.5 µg/ml. Mouse had been injected with PEG-BSA (left panels) or BSA (right panels) for 3 hours before sampling.