• All required and isotope labelled standards included

• Dried blood spot controls included

• Direct sample injection without derivatisation

• High selectivity and sensitivity by MS/MS detection

This Chromsystems reagent kit allows the very fast and reliable determination of amino acids and acylcarnitines from dried blood spot samples as part of the newborn screening for amino acid and fatty acid metabolic disorders using tandem mass spectrometry. Sample preparation is based on effective extraction of the analytes from the filter card without any further sample preparation steps required. The test utilises stable isotope labelled internal standards for calibration and measurement to ensure reliable and precise quantification of the analytes. Due to the time saving sample preparation and high precision of the MRM method, this direct injection kit stands out from the derivatised method. On the other hand lower sensitivity and interferences from isobaric substances (e.g. C5DC and C6OH) in acylcarnitines analyses may occur.

Specifications

Cutoff thresholds

LC-MS/MS parameters

LC-MS/MS system. No HPLC column is required.

Sample preparation

• Punch 3 mm dried blood spot disk out of the filter card into a well plate.
• Add 100 µl Internal Standard, agitate for 20 min.
• Inject 10 µl of the extract into the LC-MS/MS system.