β-Actin Mouse Monoclonal Antibody(6G3)

β-Actinis one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actinare useful as loading controls for Western Blotting. However it should be noted that levels of β-Actinmay not be stable in certain cells. For example, expression of β-Actinin adipose tissue is very low and therefore β-Actinshould not be used as loading control for these tissues.
Product Name:β-Actinmouse monoclonal antibody
Clone Number: 6G3
Isotype: IgG1
Immunogen: Full-length β-Actin protein of human.
Tested applications: WB, IHC. Not yet tested in other applications.
Recommended dilutions: WB: 1:5000
                         IHC: 1:200
Optimal dilutions/concentrations should be determined by the end user.
Form:Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: β-Actin antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Reactivity: Human, Rat, Mouse