Disease:

 Urinary bladder carcinoma 

Origin:

 Established from the primary bladder carcinoma (grade II) of a patient 

Species:

 Homo sapiens 

Tissue:

 Bladder (urinary) 

Morphology:

 Epithelial 

Properties:

 Adherent 

Cytogenic data:

 Tumorigenic 

Patient:

 Male, Caucasian, 68 yrs of age 

Medium:

 RPMI1640 + 10% FBS 

Subculture:

 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:

 I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Isoenzymes:
 AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1; PGM3, 2 
DNA Profile (STR):
 Amelogenin: X,Y ; CSF1PO: 11; D13S317: 11; D16S539: 9; D5S818: 11,12; D7S820: 10,11; THO1: 7,9; TPOX: 8,9; vWA: 16,18; D3S1358: 15,17; D21S11: 36; D18S51: 16,18; Penta E: 10,12; Penta D: 11; D8S1179: 10,16; FGA: 22 
Mutations in 5637 Cells 


References for 5637 cells:

1. Baierlein SA, Distel L, Sieber R, Weiss C, Rodel C, Sauer R, and Rodel F. Combined effect of tumor necrosis factor-alpha and ionizing radiation on the induction of apoptosis in 5637 bladder carcinoma cells. Strahlenther Onkol 182: 467-472, 2006.

2. Cheng G and Xie L. Parthenolide induces apoptosis and cell cycle arrest of human 5637 bladder cancer cells in vitro. Molecules 16: 6758-6768, 2010.

3. Lee SJ, Cho YH, Park K, Kim EJ, Jung KH, Park SS, Kim WJ, and Moon SK. Magnolol elicits activation of the extracellular signal-regulated kinase pathway by inducing p27KIP1-mediated G2/M-phase cell cycle arrest in human urinary bladder cancer 5637 cells. Biochem Pharmacol 75: 2289-2300, 2008.

4. Lee SJ, Cho YH, Park K, Kim EJ, Kang BS, Jung KH, Kim CH, Kim WJ, and Moon SK. Inhibitory effects of the aqueous extract of Magnolia officinalis on the responses of human urinary bladder cancer 5637 cells in vitro and mouse urinary bladder tumors induced by N-Butyl-N-(4-hydroxybutyl) nitrosamine in vivo. Phytother Res 23: 20-27, 2009.

5. Lee SJ, Kim HM, Cho YH, Park K, Kim EJ, Jung KH, Kim CH, Kim WJ, and Moon SK. Aqueous extract of Magnolia officinalis mediates proliferative capacity, p21WAF1 expression and TNF-alpha-induced NF-kappaB activity in human urinary bladder cancer 5637 cells; involvement of p38 MAP kinase. Oncol Rep 18: 729-736, 2007.

6. Lee SJ, Park SS, Lee US, Kim WJ, and Moon SK. Signaling pathway for TNF-alpha-induced MMP-9 expression: mediation through p38 MAP kinase, and inhibition by anti-cancer molecule magnolol in human urinary bladder cancer 5637 cells. Int Immunopharmacol 8: 1821-1826, 2008.

7. Mao Q, Li Y, Zheng X, Yang K, Shen H, Qin J, Bai Y, Kong D, Jia X, and Xie L. Up-regulation of E-cadherin by small activating RNA inhibits cell invasion and migration in 5637 human bladder cancer cells. Biochem Biophys Res Commun 375: 566-570, 2008. 

8. Matin SF, Rackley RR, Sadhukhan PC, Kim MS, Novick AC, and Bandyopadhyay SK. Impaired alpha-interferon signaling in transitional cell carcinoma: lack of p48 expression in 5637 cells. Cancer Res 61: 2261-2266, 2001. 

9. Mills M, Meysick KC, and O'Brien AD. Cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli kills cultured human uroepithelial 5637 cells by an apoptotic mechanism. Infect Immun 68: 5869-5880, 2000. 

10. Monami G, Gonzalez EM, Hellman M, Gomella LG, Baffa R, Iozzo RV, and Morrione A. Proepithelin promotes migration and invasion of 5637 bladder cancer cells through the activation of ERK1/2 and the formation of a paxillin/FAK/ERK complex. Cancer Res 66: 7103-7110, 2006. 

11. Plattner VE, Wagner M, Ratzinger G, Gabor F, and Wirth M. Targeted drug delivery: binding and uptake of plant lectins using human 5637 bladder cancer cells. Eur J Pharm Biopharm 70: 572-576, 2008. 

12. Rassouli FB, Matin MM, Iranshahi M, Bahrami AR, Behravan J, Mollazadeh S, Neshati V, and Kalalinia F. Investigating the enhancement of cisplatin cytotoxicity on 5637 cells by combination with mogoltacin. Toxicol In Vitro 25: 469-474, 2010.