简介
GenJet™体外DNA转染试剂是一种新结构类型的DNA转染试剂。该转染试剂为阳离子聚合物和脂质体的杂合体，具有转染效率高、重复性好、毒性低和稳定性极强的特点(室温条件下可保存18个月!)，目前该DNA转染试剂为我公司独家产品。GenJet™转染试剂用于哺乳动物细胞的转染，如肿瘤细胞系(常见的细胞有HEK293, CHO, COS-1, COS-7, Hela, NIH3T3等)和原代培养的细胞以及昆虫细胞sf9, sf21等。GenJet™试剂，1.0 ml，可用于24孔板转染333次或者6孔板166次转染操作。
应用
- 将DNA导入哺乳动物细胞和昆虫细胞
- 同病毒协同转染难转哺乳动物细胞，可显著增强病毒的转染效率(可增高200倍)
特点
- 对哺乳动物细胞有极高的转染效率
- 可获得极高滴度的病毒(如慢病毒， AAV等)
- 对大片段DNA分子(大到180 kb)有同样高的转染效率
- 对单个DNA或多个DNA共转染具有同样好的效率
- 可获得高水平的重组蛋白表达
- 不受血清和抗生素的影响
- 对难转原代细胞有较好的转染效率
保存条件
该产品于4~23°C保存，可稳定18个月。
GenJet™转染试剂哺乳动物细胞转染谱
| Cell Lines |
Transfection Efficiency (% GFP) |
Cell Lines |
Transfection Efficiency (% GFP) |
3LL
B16-F10
BAEC
BHK-21
Ca Ski
CaCo-2
CHO
HCS-2/8
HEK-293
HeLa
HLMEC
H-MVEC
Huh-7D12
ATT20
SK-N-SH
McArdle 7777
Hep3D
SHEP
MDA-MB-231
3T3-442A
COS-7
CV-1
D 407
DHD Pro.b
LS180 |
51%
72%
52%
86%
78%
32%
83%
42%
80-90%
81%
50-65%
49%
22%
38%
20%
60%
78%
71%
38%
10%
60-70%
53%
62%
51%
46% |
K562
L929
MCF-7
MDCK
Neuro2A
NIH 3T3
PC12
SH-SY5Y
SiHa
SKOV3
HUVEC
IGROV1
Jurkat
6CSFMEo
WEHI 231
SAOS-2
SN56
MC3T3-E1
BT474
C2C12
Primary melanocyte
Hela-S3
A549
Hep G2 |
22%
52%
51%
52%
72%
88%
34%
14%
62%
52%
51%
21%
5%-15%
73%
25%
52%
69%
70%
46%
46%
46%
78%
78%
58% |
GenJet体外DNA转染试剂同市场上知名品牌的比较
Exceptional high titers of lentivirus generated from 293F cells. Three DNAs co-transfected with GenJet™ In Vitro DNA Transfection Reagent (left panel) in comparison of LipoFectamine 2000™ (L2K, right panel). 100 microliter virus supernatant from 293F cells were used to counter-infected 293F cells which was then passed FACS. The numbers at the upper right corner indicate the percentage of transduced cells. The titers of lentivirus generated with GenJet™ and L2K were quantified to be 7.9x106 and 3.1x106 tu/ml respectively
Primary Murine Skin Fibroblast transfected with pEGFP-C1 plasmid using GenJet™ In Vitro DNA Transfection Reagent (upper panel) and the most popular brand product FuGene™ HD (lower panel). The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection
Primary Murine Keratinocytes transfected with pEGFP-C1 plasmid using GenJet™ In Vitro DNA Transfection Reagent (upper panel) and one of the most popular brand product FuGene™ HD (lower panel). The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection
Comparison of cytotoxicity of GenJet™ DNA In Vitro Transfection Reagent with L2K™ on primary murine skin fibroblast. The primary murine fibroblast was incubated with the indicated transfection reagents/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post transfection
Technical Information & Datasheet
- GenJet™ In Vitro DNA Transfection Reagent FAQ
- GenJet™ In Vitro DNA Transfection Insert Sheet
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