SPECIFICATION SHEET

Catalog #:                         L1C00801              Lot #:         C080401

Description:                    MAb to Cancer Antigen CA15-3

                                                Monoclonal Antibody to Cancer Antigen CA15-3 (Breast Cancer)

Specificity:                       recognizes a sialylated carbohydrate epitope of the MUC-1 glycoprotein. Is sensitive to periodate and sialidase treatment, but it does not react with glycolipid extract from the ZR 75-1 cell line. The MUC-1 mucin (Cancer Antigen 15-3) is secreted from tumor cells. The apo-protein of the MUC-1 mucin contains a transmembrane domain, a cytoplasmic domain, and an extracellular carbohydrate rich domain. The extracellular domain is characterized by polymorphism with respect to the number of a 20 amino acid tandem repeat (VNTR polymorphism).

Clone:                                 ML04H001

Host Animal:                  Mouse. Hybridization of P3X63-Ag8.653                                     

Isotype:                             IgG_1, myeloma cells with spleen cells from Balb/c mice.

Source:                              Mouse Ascites

Immunogen:                   Human breast cancer cell line ZR 75-1

Format:                              Purified, Liquid

Purification:                  Protein A chromatography

Concentration:             3.0mg/ml (OD280nm)

Affinity Constant:     Not determined

Buffer:                               150mM NaCl 10mM Tris  PH7.4

Preservative:                  0.05%NaN3

Applications:                 Capture or detection antibody in serological assays for the research of antigen MUC-1. Forms a suitable pair with ML04H002, Catalog # L1C00802. Immunohistochemistry on frozen or paraffin sections for the detection of MUC-1. Immunochemical detection of MUC-1 protein. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Storage:                             Short-term (up to 2 months) store at <-20°C. Long term (>2 months) store at <-40°C.  Avoid multiple freeze/thaw cycles.