• 偶联物Alexa Fluor® 647. Ex: 652nm, Em: 668nm
• 经测试应用IHC-P, ICC/IFmore details
• 种属反应性
  与反应: Mouse, Rat, Human
• 免疫原

  Synthetic peptide within Human NeuN aa 1-100 (Cysteine residue). The exact sequence is proprietary.
  Database link: A6NFN3

• 阳性对照
  • This antibody gave a positive signal in the folowing tissue lysates: Mouse Brain; Mouse Cerebellum; Rat cerebellum; Human Fetal brain as well as the following whole cell lysates: 293T, HeLa and SH-SY5Y lysates; SH-SY-5Y cells. ICC/IF - NGF-differentiated PC12 cells and U87-MG cells
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  The Alexa Fluor^® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor^® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor^® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor^® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

   

  Alternative versions available:

  Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  Anti-NeuN antibody (Alexa Fluor^® 488) [EPR12763] - Neuronal Marker (ab190195)

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
• 存储溶液pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 68% PBS, 30% Glycerol, 1% BSA
• 浓度100 µl 浓度为 0.5 mg/ml 
  
• 纯度Affinity Purified
• 克隆单克隆
• 克隆编号EPR12763
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Cell Type Marker
   
  • Neuron marker
   
  • Soma marker

  • Tags & Cell Markers
   
  • Cell Type Markers
   
  • Neuroscience Markers
   
  • Neuronal

  • Epigenetics and Nuclear Signaling
   
  • Transcription
   
  • Domain Families
  • Forkhead Box
   
  • Other FOXes

  • Epigenetics and Nuclear Signaling
   
  • Transcription
   
  • Transcription Factors

应用

靶标

• 功能RNA-binding protein that regulates alternative splicing events.
• 序列相似性Contains 1 RRM (RNA recognition motif) domain.
• 细胞定位Nucleus. Cytoplasm.

• Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 647) 图像

  • 

    Immunocytochemistry/ Immunofluorescence - Anti-NeuN [EPR12763] antibody - Neuronal Marker (Alexa Fluor® 647) (ab190565)

    ab190565 staining NeuN in NGF-differentiated PC12 cells (7 days). The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190565 at 1/50 dilution (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor^® 488 Goat anti-Mouse secondary (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

     

  • 

    Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 647) (ab190565)

    ab190565 staining NeuN in U87-MG cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190565 at 1/50 dilution(shown in red) and ab7291(Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor^® 488 Goat anti-Mouse secondary (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 647) (ab190565)

    IHC image of ab190565 staining in formalin fixed paraffin embedded tissue section of normal human cerebellum.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab190565 (1/50) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then counterstained and mounted with SlowFade^® Gold Antifade Mountant with DAPI.

    TUNEology 波长可调检测卡盒-->