人MMP抗体阵列-膜(10 Targets)-抗体相关-抗体-生物在线

产品名称：人MMP抗体阵列-膜(10 Targets)

英文名称：人MMP抗体Array -膜(10 Targets) (ab134004)

产品编号： ab134004

产品价格： null

产品产地：英国

品牌商标： abcam

更新时间： null

使用范围：

深圳市宇德立生物科技有限公司

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样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
检测类型Semi-quantitative
种属反应性
与反应: Human
产品概述

ab134004 is for simultaneous detection of 7 Human MMPs and 3 TIMPs. Suitable for all sample types.

Targets: MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4

Learn more about membrane antibody arrays
经测试应用Multiplex Protein Detectionmore details

组件	1 x 4 Membranes	1 x 8 Membranes
1000X HRP-Conjugated Streptavidin	1 x 50µl	1 x 50µl
1X Blocking Buffer	1 x 25ml	1 x 50ml
20X Wash Buffer I	1 x 10ml	1 x 20ml
20X Wash Buffer II	1 x 10ml	1 x 20ml
2X Cell Lysis Buffer	1 x 10ml	1 x 20ml
8-Well Plastic Tray	1 unit	1 unit
Biotin-Conjugated Anti-Cytokines	2 vials	4 vials
Cytokine Antibody Array Membranes	4 units	8 units
Detection Buffer C	1 x 1.5ml	1 x 2.5ml
Detection Buffer D	1 x 1.5ml	1 x 2.5ml

Our Abpromise guarantee covers the use of ab134004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Abreviews	说明
Multiplex Protein Detection		Use at an assay dependent concentration.

Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)

Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004.
Quantification of Human MMP Antibody Array

Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.
Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)

143B cells (Human osteosarcoma) were seeded at 1x10⁶ cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.

Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134004.
Quantification of Human MMP Antibody Array

Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.
Dot Blot - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)This image is courtesy of an anonymous customer