预优化DNA转染试剂-WEHI-231-克隆与表达-试剂-生物在线
济南思科生物科技有限公司
预优化DNA转染试剂-WEHI-231

预优化DNA转染试剂-WEHI-231

商家询价

产品名称: 预优化DNA转染试剂-WEHI-231

英文名称: GenJet™ In Vitro DNA Tranfection Reagent for WEHI-231 Cells

产品编号: SL100489-WEHI

产品价格: 0

产品产地: 美国

品牌商标: SignaGen

更新时间: null

使用范围: null

济南思科生物科技有限公司
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  • 地址 : 济南市经六路689号绿地·玫瑰坊A座734号
  • 邮编 : 250021
  • 所在区域 : 山东
  • 电话 : 159****9896 点击查看
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  • 邮箱 : jnqsw@163.com; info@signagen-china.com

Description

GenJet™ DNA In Vitro Tranfection Reagent for WEHI-231 is pre-optimized for transfecting WEHI-231 cells.  WEHI-231 is a murine B cell lymphoma that has been used extensively as a model for the immature stage B cell and its functional response to Ag receptor cross-linking as a model for immature B cell tolerance. This cell line expresses sIgM, CD5, and FcR gamma, but lacks the B cell- specific isoform of CD45 (B220).

Refer to the following optimal transfection conditio
ns for maximal transfection efficiency on WEHI-231
 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency 

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


~95%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours


EGFP
pEGFP-N3 (CMV promoter) 

66%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet.pdf