重组人TIGIT蛋白（Recombinant Human TIGIT Protein），GMP级

产品货号：GMP-TL730

产品描述（Description）

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

分子别名（Synonym）

VSIG9、VSTM3

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列编码人TIGIT（NCBI Reference Sequence:NP_776160.2）表达带有Fc标签在C末端

分子量（Molecular Weight）

重组人TIGIT蛋白 包含351个氨基酸，预测理论分子量为40kD

纯度（Purity）

≥95%采用SDS-PAGE凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人TIGIT(His标签)1ug/mL(100 uL/孔)你能够结合人CD155（His 标签)的线性范围是10-100 ng/mL

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、TIGIT Marks Exhausted T Cells, Correlates with Disease Progression, and Serves as a Target for Immune Restoration in HIV and SIV Infection Glen M. Chew, Tsuyoshi Fujita, Gabriela M. Webb, Benjamin J. Burwitz, Helen L. Wu, Jason S. Reed, Katherine B. Hammond, Kiera L. Clayton, Naoto Ishii, Mohamed Abdel-Mohsen, Teri Liegler, Brooks I. Mitchell, Frederick M. Hecht, Mario Ostrowski, Cecilia M. Shikuma, Scott G. Hansen, Mark Maurer, Alan J. Korman, Steven G. Deeks, Jonah B. Sacha, Lishomwa C. Ndhlovu PLoS Pathog. 2016 Jan; 12(1): e1005349. Published online 2016 Jan 7. doi: 10.1371/journal.ppat.1005349 

2、TIGIT predominantly regulates the immune response via regulatory T cells Sema Kurtulus, Kaori Sakuishi, Shin-Foong Ngiow, Nicole Joller, Dewar J. Tan, Michele W.L. Teng, Mark J. Smyth, Vijay K. Kuchroo, Ana C. Anderson J Clin Invest. 2015 Nov 2; 125(11): 4053–4062. Published online 2015 Sep 28. doi: 10.1172/JCI81187

  注意：For research and manufacturing use.