ConA 诱导的急性炎症模型
产品名称: ConA 诱导的急性炎症模型
英文名称: Con A induced IL-2 release
产品编号: BDA-IF001
产品价格: 询价
产品产地: null
品牌商标: null
更新时间: null
使用范围: null
上海百代安生物科技有限公司
- 联系人 :
- 地址 : 上海市浦东新区张江高科蔡伦路720号1号楼413室
- 邮编 : 201203
- 所在区域 : 上海
- 电话 : 186****6750 点击查看
- 传真 : 点击查看
- 邮箱 : kyh@gmail.com
ConA 诱导的急性炎症模型
实验方案:
1) 无菌生理盐水配置ConA溶液 (1mg/ml), 冰上放置;
2) 给药后一小时尾静脉注射ConA溶液;
3) ConA注射1个半小时后取血,肝素抗凝;
4) 离心后血浆检测IL-2等细胞因子。
Brief protocol of ConA induced IL-2 release assay
1) 1 mg/ml of Con A was suspended in sterile saline. Store the Con A solution in ice.
2) One hour following compound administration (PO), mice are given an IV injection of Con A (10mg/kg)
3) Ninety minutes after the Con A injection, blood was collected in a Heparin sodium containing tube.
4) Blood samples were spun at 3,000 rpm at 4℃ for 10 minutes. Plasma were stored in -80℃ freezer until further analysis.