疏水层析Cellufine Butyl/Phenyl-色谱介质-耗材-生物在线
智索国际贸易有限公司
疏水层析Cellufine Butyl/Phenyl

疏水层析Cellufine Butyl/Phenyl

商家询价

产品名称: 疏水层析Cellufine Butyl/Phenyl

英文名称: Cellufine Butyl/Phenyl

产品编号: 19905/19900

产品价格: 0

产品产地: CHISSO Japan

品牌商标: Cellufine Butyl/Phenyl

更新时间: null

使用范围: null

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Introduction

 

For purification of proteins and macromolecules

 

Hydrophobic Interaction Chromatography (HIC) is a method which separates proteins on the basis of their differential interactions with a mildly hydrophobic surface. HIC media are porous chromatography particles, manufactured from crosslinked cellulose to which either a butyl, phenyl or octyl functionality has been covalently bonded via a short spacer. Factors which affect hydrophobic interactions include: salt concentrations, temperature, pH, surfactant and organic solvents. Usually the higher the ionic strength (salt concentration) the stronger the hydrophobic bond. Consequently the interaction is enhanced by conditions inverse to that of ion exchange chromatography. HIC is, therefore, an effective complementary tool for separating and purifying substances which are difficult or cannot be separated by ion exchange.

 

Features

 

 Spherical particles exhibiting high mechanical strength • Butyl and Phenyl functionality • Pre-swollen • Virtually no shrinkage or swelling • Stable in organic solvents and surfactants • Stable coupling chemistry • Resistant to 0.2 M NaOH • Autoclavable (121 °C, 20 min)

 

Benefits

 

High flow rates allowing rapid chromatography and direct scale-up • Enables optimum selectivity to be obtained • Easy packing • Easy large scale operation. No shrinkage at high salt concentrations • Enables range of solvent systems to be utilized • Resistant to cleaning and elution conditions • Sterilizable • Regulatory support