重组人LAG-3(Fc)蛋白（Recombinant Human LAG-3(Fc) Protein），GMP级

产品货号：GMP-TL749

产品描述（Description）

Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains.

The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal

localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in

lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.

分子别名（Synonym）

CD223、FDC

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA 序列编码人 LAG-3（NCBI Reference Sequence:NP_001762.2 ）表达带有 Fc 标签在 C 末端

分子量（Molecular Weight）

重组人LAG-3蛋白包含 689 个氨基酸，预测理论分子量为 75.6kD

纯度（Purity）

≥95%采用SDS-PAGE凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人 LAG-3(Fc 标签)的抗体 5μg/mL (100 μL/孔)能够结合人 FGL-1(His 标签) 的线性范围是 50-200ng/mL

提纯方法（ Purification Method ）

层析纯化

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、 LAG-3 confers a competitive disadvantage upon antiviral CD8+ T cell responses Kevin D. Cook, Jason K. Whitmire J Immunol. Author manuscript; available in PMC 2017 Oct 30. Published in final edited form as: J Immunol. 2016 Jul 1; 197(1): 119–127. Published online 2016 May 20. doi: 10.4049/jimmunol.1401594 

2、The promising immune checkpoint LAG-3: from tumor microenvironment to cancer immunotherapy Long Long, Xue Zhang, Fuchun Chen, Qi Pan, Pronnaphat Phiphatwatchara, Yuyang Zeng, Honglei Chen Genes Cancer. 2018 May; 9(5-6): 176–189. doi: 10.18632/genesandcancer.180 

Lymphocyte Activation Gene 3 (LAG-3) Modulates the Ability of CD4 T-cells to Be Suppressed In Vivo Nicholas M. Durham, Christopher J. Nirschl, Christopher M. Jackson, Jimmy Elias, Christina M. Kochel, Robert A. Anders, Charles G. Drake PLoS One. 2014; 9(11): e109080. Published online 2014 Nov 5. doi: 10.1371/journal.pone.0109080

  注意：For research and manufacturing use.