The Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator™ provides a hassle-free method for the rapid purification and concentration of high quality DNA from any step in a “standard” ChIP protocol. This includes samples that have undergone reverse cross-linking, Proteinase K or RNase A digestion, mechanical or nuclease-mediated DNA shearing, and samples eluted from chromatin-antibody-bead complexes. Additionally, this product may also be used to purify DNA from PCR and other enzymatic reactions.

The specially formulated ChIP DNA Binding Buffer promotes DNA adsorption to the column in the presence of detergents, antibodies, and proteinases that are often used for ChIP. Simply add the ChIP DNA Binding Buffer to your sample and transfer the mixture to the supplied Zymo-Spin™ Column. There is no need for organic extraction or ethanol precipitation. Instead, Fast-Spin column technology yields ultra-pure DNA in just minutes. The DNA purified using the ChIP DNA Clean & Concentrator™ is ideal for PCR amplification, arrays, DNA quantification, as well as other molecular applications. This kit may be applied to any routine ChIP procedure to determine DNA concentration of samples that have undergone reverse cross-linking following DNA shearing. It can also be used for the removal of TES, 0.1M NaHCO3, and 1% SDS from DNA eluted from chromatin-antibody-bead complexes and can be used to purify DNA from buffers containing up to 1% SDS or 5% NP-40, Tween-20, Triton X-100 or Sarkosyl.

The ChIP DNA Clean & Concentrator™ recovers ultra-pure DNA from cell lysates that is proportional to the lysate volume and DNA fragment size range (see ptotocol ).

Overview of ChIP DNA Clean & Concentrator™ Procedure