半胱氨酸蛋白酶抗体-抗体-抗体-生物在线
上海雅吉生物科技有限公司
半胱氨酸蛋白酶抗体

半胱氨酸蛋白酶抗体

商家询价

产品名称： 半胱氨酸蛋白酶抗体

英文名称： HSD17B3

产品编号： 3905

产品价格： null

产品产地： 上海

品牌商标： 雅吉

更新时间： null

使用范围： WB ELISA IHC-P Flow-Cyt

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中文名称 半胱氨酸蛋白酶抗体
别    名 AEP; Asparaginyl endopeptidase; EC 3.4.22.34; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1 (legumain); PRSC1; LGMN_HUMAN.  
研究领域 肿瘤  细胞生物  免疫学  细胞凋亡  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Dog, Cow, Horse, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 Flow-Cyt=3ug/Test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 46kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Legumain:201-300/433 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008].

Function:
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Subcellular Location:
Lysosome (By similarity).

Tissue Specificity:
Ubiquitous. Particularly abundant in kidney, heart and placenta.

Post-translational modifications:
Glycosylated.

Similarity:
Belongs to the peptidase C13 family.

SWISS:
Q99538

Gene ID:
5641

Database links:

Entrez Gene: 5641 Human

Entrez Gene: 19141 Mouse

Entrez Gene: 63865 Rat

Omim: 602620 Human

SwissProt: Q99538 Human

SwissProt: O89017 Mouse

SwissProt: Q9R0J8 Rat

Unigene: 726036 Human

Unigene: 17185 Mouse

Unigene: 206021 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-Legumain (bs-3907R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 55 kD
Sample: Kidney (Mouse) Lysate at 40 ug
Primary: Anti- Legumain (bs-3907R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 55 kD
Blank control: HL60. Primary Antibody (green line): Rabbit Anti-Legumain antibody (bs-3907R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Overlay histogram showing Mouse spleen cells stained with bs-3907-PE (red line). The cells were fixed with 1% paraformaldehyde (10 min).The cells were then incubated with the antibody (bs-3907R-PE, 2ug/1x106cells) for 30 min at 22-25℃. Isotype control antibody (black line) was rabbit IgG (2ug/1x106cells) used under the same conditions. Acquisition of events were used for analysis.