研究领域 肿瘤  信号转导  合成与降解  新陈代谢   

抗体来源 Rabbit 

克隆类型 Polyclonal 

交叉反应 Human, Mouse,  (predicted: Pig, Horse, Rabbit, Guinea Pig, ) 

产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user. 

分 子 量 31kDa 

细胞定位 细胞浆  

性    状 Liquid 

浓    度 1mg/ml 

免 疫 原 KLH conjugated synthetic peptide derived from human NQO1:201-274/274  

亚    型 IgG 

纯化方法 affinity purified by Protein A 

储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 

保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 

PubMed PubMed 

产品介绍 This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Function:

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

Subunit:

Homodimer.

Subcellular Location:

Cytoplasm.

Similarity:

Belongs to the NAD(P)H dehydrogenase (quinone) family.

SWISS:

P15559

Gene ID:

1728

合成与降解（Synthesis and Degradation）

醌氧化还原酶（NQO1）是一种黄素酶，它催化醌双电子还原反应，以减少氧自由基的产生。经研究发现NQO1可能有抗氧化效应，目前多用于帕金森病（PD）和部分肿瘤方面的研究。

醌是一种有毒的化合物,能诱发哺乳动物细胞癌变、突变和坏死。

Database links:

Entrez Gene: 610935 DogEntrez Gene: 1728 HumanEntrez Gene: 18104 MouseEntrez Gene: 100286873 PigEntrez Gene: 24314 RatOmim: 125860 HumanSwissProt

产品图片

Sample: Lane1: Liver(Rat) Lysate at 30 ug Lane2: Brain(Rat) Lysate at 30 ug Primary: Anti-NQO1 (bs-2184R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 31kD Observed band size : 30kD Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-NQO1 (bs-2184R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD Protein: A549 lysate at 30ug; Primary: Anti-NQO1 (bs-2184R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size : 31 kD Observed band size :31 kD Tissue/cell: mouse colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NQO1 Polyclonal Antibody, Unconjugated(bs-2184R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining