兔抗人表皮脂肪酸结合蛋白多克隆抗体
产品名称: 兔抗人表皮脂肪酸结合蛋白多克隆抗体
英文名称: Rabbit Anti-human Epidrmal Fatty Acid Binding Protein (EFABP) IgG, PAb
产品编号: RD004-02
产品价格: null
产品产地: BIOVENDOR
品牌商标: BIOVENDOR
更新时间: null
使用范围:
- 联系人 :
- 地址 : 上海市浦东新区国际医学园区康新公路3399弄3号楼
- 邮编 : 201203
- 所在区域 : 上海
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- 邮箱 : marketing@majorbio.com
规格: 0.1 mg (A280 测定, 与BSA的相关系数为0.704)
抗原:重组人EFABP(在大肠杆菌中表达),与人血清中的EFABP氨基酸序列完全相同。
宿主: 兔
滴度:>1:100,000 (1mg/ml)。间接ELISA方法测定,每孔包被25ng抗原。
纯化方法:免疫亲和层析纯化。
产品形态:冻干粉(无菌过滤,1 mg/ml,PBS缓冲液)
溶解:加0.1ml去离子水,室温下彻底溶解,最终浓度为1 mg/ml。若有轻微混浊,不影响抗体活性,使用前离心即可。
保存条件: 冻干粉-20℃保存,溶解后-80℃保存。建议溶解后分装保存,避免反复冻融。溶解后可以暂时保存在4℃至两周。
应用: ELISA、Western blotting。
Size: 0.1 mg (determined by absorbance of A280 and the correlation coefficient against BSA is 0.704).
Antigen: Recombinant human EFABP produced in E. coli. The recombinant human EFABP is 100% homologous with the human serum EFABP.
Host: Rabbit
Titer: Is defined by indirect ELISA, it is >1:100,000 for antibody concentration 1mg/ml, 25 ng of antigen is coated per well, and then defined at a point of maximal decrease of the titration curve.
Purification Method: Immunoaffinity chromatography on a column with immobilized human recombinant EFABP.
Formulation: Sterile filtered and lyophilized from 1 mg/ml in 0.1 M phosphate buffer, 0.1 M NaCl, pH 7.2.
Reconstitution: Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage: Store lyophilized antibody at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.
Stability/Shelf Life: The lyophilized antibody remains stable and fully active until the expiry date when stored at -20°C.
Applications: ELISA, Western blotting.