• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• 纯度Whole antiserum
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Neurology process
   
  • Neurodegenerative disease
  • Alzheimer's disease
   
  • Other

  • Neuroscience
   
  • Cell Adhesion Proteins
   
  • Cytoskeletal Proteins
  • Intermediate Filaments

  • Signal Transduction
   
  • Cytoskeleton / ECM
   
  • Cytoskeleton
  • Intermediate Filaments
   
  • Class IV
   
  • Neurofilaments

  • Neuroscience
   
  • Neurology process
   
  • Neurodegenerative disease
   
  • Other

  • Neuroscience
   
  • Cell Type Marker
   
  • Neuron marker
   
  • Axon marker

应用

靶标

• 功能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
• 疾病相关Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
• 序列相似性Belongs to the intermediate filament family.
• 翻译后修饰There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
  Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
  Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
• Information by UniProt
• 数据库链接
  • Entrez Gene: 528842 Cow
  • Entrez Gene: 4744 Human
  • Entrez Gene: 380684 Mouse
  • Entrez Gene: 100156492 Pig
  • Entrez Gene: 24587 Rat
  • Omim: 162230 Human
  • SwissProt: P12036 Human
  • SwissProt: P19246 Mouse

  see all
• 别名
  • 200 kDa neurofilament protein antibody
  • Nefh antibody
  • Neurofilament heavy polypeptide 200kDa antibody

  see all

Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker 图像

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-200 kD Neurofilament Heavy antibody - Neuronal Marker (ab8135)
  The image showing results obtained after protease pretreatment, illustrating high definition neurofilament staining. This picture was kindly supplied as part of the review submitted by Prof Colm Cunningham and Dr Suzanne Campion (Southampton University).
• 

  Immunohistochemistry (Frozen sections) - 200 kD Neurofilament Heavy antibody - Neuronal Marker (ab8135)This image is courtesy of an anonymous Abreview

  ab8135 at 1/500 staining aged muscle tissue sections from Rat by IHC-Fr.

  The tissue section was permeabilized with Triton X100 and blocked with 10% serum for 30 minutes at 25°C.

  The primary antibody was incubated for 1 hour at 25°C.

  Muscle was stained without fixation and post-fixed in 10% NBF following staining for 1h.

  Green = alpha bungarotoxin stained post-synaptic neuromuscular junction.

  Red = ab8135 staining 200kD Neurofilament

   

  TUNEology 波长可调检测卡盒-->