• 经测试应用WB, ICC/IFmore details
• 种属反应性
  与反应: Human
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Sts1 aa 1-100. The exact sequence is proprietary.
  Database link: Q8TF42

• 阳性对照
  • HepG2 and Jurkat whole cell lysates; Jurkat cells.
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
• 浓度100 µl 浓度为 0.56 mg/ml 40 µl 浓度为 0.56 mg/ml 10 µl 浓度为 0.56 mg/ml 
  
• 纯度Protein A purified
• 克隆单克隆
• 克隆编号EPR16980
• 同种型IgG
• 研究领域
  • Immunology
   
  • Adaptive Immunity
   
  • T Cells
   
  • Cytotoxic Cells

  • Cell Biology
   
  • Proteolysis / Ubiquitin
   
  • Proteasome / Ubiquitin
   
  • Ubiquitin

  • Signal Transduction
   
  • Protein Trafficking
   
  • Vesicle Transport
   
  • Adapters

应用

靶标

• 功能Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.
• 序列相似性Contains 1 SH3 domain.
  Contains 1 UBA domain.
• 细胞定位Cytoplasm. Nucleus.
• Information by UniProt
• 数据库链接
  • Entrez Gene: 84959 Human
  • Omim: 609201 Human
  • SwissProt: Q8TF42 Human
  • Unigene: 444075 Human
• 别名
  • Cbl interacting protein p70 antibody
  • Cbl interacting protein Sts 1 antibody
  • Cbl interacting protein Sts1 antibody

  see all

Anti-Sts1 antibody [EPR16980] 图像

• 

  Western blot - Anti-Sts1 antibody [EPR16980] (ab197027)
  All lanes : Anti-Sts1 antibody [EPR16980] (ab197027) at 1/2000 dilution
  
  Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
  Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size : 73 kDa
  Observed band size : 73 kDa
  
  
  Exposure time : 3 minutes

  Blocking/Dilution buffer: 5% NFDM/TBST.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Sts1 antibody [EPR16980] (ab197027)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Sts1 with ab197027 at 1/280 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm and weakly nuclear staining on Jurkat cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
  The negative controls are as follows;
  -ve control 1: ab197027 at 1/280 dilution followed byab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
  -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.