微管相关蛋白1A抗体-抗体-抗体-生物在线
上海雅吉生物科技有限公司
微管相关蛋白1A抗体

微管相关蛋白1A抗体

商家询价

产品名称： 微管相关蛋白1A抗体

英文名称： MAP1A

产品编号： 1847

产品价格： null

产品产地： 上海

品牌商标： 雅吉

更新时间： null

使用范围： IHC-P IHC-F Flow-Cyt IF

上海雅吉生物科技有限公司
  • 联系人 :
  • 地址 : 上海市闵行区元江路5500号第1幢5658室
  • 邮编 :
  • 所在区域 : 上海
  • 电话 : 158****3937 点击查看
  • 传真 : 点击查看
  • 邮箱 : yajikit@163.com
进入展台

 

中文名称 微管相关蛋白1A抗体
别    名 MAP 1A; MAP-1A; MTAP1A; MAP 1L; MAP1L; MAP1A heavy chain; Map1a; MAP1A_HUMAN; Microtubule associated protein 1 like; Microtubule Associated Protein 1A; Microtubule-associated protein 1A; MTAP1A; Proliferation related protein p80; Proliferation-related protein p80; AI853608; Map1a; moth1; Mtap-1; Mtap1.  
研究领域 肿瘤  细胞生物  免疫学  神经生物学  信号转导  细胞凋亡  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat,  (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, )
产品应用 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 326kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MAP1A heavy chain:2651-1750/3014 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1A heavy chain and LC2 light chain. Expression of this gene is almost exclusively in the brain. Studies of the rat microtubule-associated protein 1A gene suggested a role in early events of spinal cord development. [provided by RefSeq, Jul 2008]

Function:
Structural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements.

Subunit:
3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Interacts with TIAM2. Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG4. Binds to CSNK1D.

Subcellular Location:
Cytoplasm, cytoskeleton (Probable).

Tissue Specificity:
Brain

Post-translational modifications:
Phosphorylated by CSNK1D.
LC2 is generated from MAP1A by proteolytic processing.

Similarity:
Belongs to the MAP1 family.

SWISS:
P78559

Gene ID:
4130

Database links:

Entrez Gene: 4130 Human

Entrez Gene: 17754 Mouse

Entrez Gene: 25152 Rat

Omim: 600178 Human

SwissProt: P78559 Human

SwissProt: Q9QYR6 Mouse

SwissProt: P34926 Rat

Unigene: 194301 Human

Unigene: 619338 Human

Unigene: 36501 Mouse

Unigene: 11402 Rat

Unigene: 41412 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP1A) Polyclonal Antibody, Unconjugated (bs-1847R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MAP1A Polyclonal Antibody, Unconjugated(bs-1847R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MAP1A Polyclonal Antibody, Unconjugated(bs-1847R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): U251 (blue).
Primary Antibody (green line): Rabbit Anti- MAP1A antibody (bs-1847R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min , then permeabilized) with 90% ice-cold methanol for 20 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

​