抗酪氨酸磷酸化兔多克隆泛抗体
产品名称： 抗酪氨酸磷酸化兔多克隆泛抗体
英文名称： Pan anti-phosphotyrosine antibody agarose conjugated beads
产品编号： PTM-702
产品价格： null
产品产地： 杭州(Hangzhou China)
品牌商标： PTM
更新时间： null
使用范围： WB IP
杭州景杰生物科技股份有限公司
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Pan anti-phosphotyrosine rabbit pAb Cat#PTM-703
| Immunogen: Phosphotyramine-KLH Reactivity: All species expected Product Description: Anti-phosphotyrosine rabbit polyclonal antibody was developed by using phosphotyramine-KLH conjugates as immunogen. This antibody can be used in western blotting to examine the modification of tyrosine phosphorylation in immunoprecipitated proteins or the overall profile of tyrosine phosphorylation in cells and tissues. Source/Purification: The rabbit polyclonal antibody is produced by immunizing animals with synthetic phospho-Tyr-containing peptides and immunoaffinity purified by protein A followed by phosphotyramine affinity chromatography. Specificity: Anti-phosphotyrosine rabbit pAb is a high affinity antibody selectively against phosphorylated tyrosine. ELISA and dot blot assay using a wide variety of phospho-peptides indicate that this antibody only binds to tyrosine-phosphorylated peptides independent of the surrounding amino acid sequence but not to threonine, serine or unmodified peptides. Western blotting analysis on crude proteins extracted from HeLa cells shows that this antibody interacts with a broad range of tyrosine-phosphorylated proteins induced by sodium vanadate treatment. Recommended Applications: ELISA, WB, IF, Not test in other applications *WB=Western blotting; IP=Immunopreciptation; IHC=Immunohistochemistry. Recommended dilution: WB:1:1000; IP: 1:200; IHC: 1:200. IMPORTANT: For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight. Use at an assay dependent concentration.Optimal dilutions/concentrations should be determined by the end user and maybe assay dependent. Not yet tested in other applications.  A. Dot blotting analysis on 20 ng of tyrosine phosphorylated peptide library (1A), site specific tyrosine phosphorylated peptides (2A-5A,1B-5B and 1C-4C), threonine phosphorylated peptides (5C and 6A-6C), serine phosphorylated peptides (7A-7C and 8A) and unmodified peptides (8B-8C) using anti-phosphotyrosine rabbit polyclonal antibody. B. Western blotting analysis on 30 μg of crude proteins extracted from HeLa cells with (right) or without (left) sodium vanadate treatment (1 mM for 15 mins) using anti-phosphotyrosine rabbit polyclonal antibody (1:1000). | ||
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Scientific Description: Tyrosine phosphorylation is one of the major means of cell signaling transduction in multiple pathophysiological conditions regulated by tyrosine kin | ||