PDE8A polyclonal antibody
产品名称： PDE8A polyclonal antibody
英文名称： PDE8A polyclonal antibody
产品编号： PAB1878
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PDE8A.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to internal region of human PDE8A.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein A purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Per-Arnt-Sim domain-dependent association of cAMP-phosphodiesterase 8A1 with IkappaB proteins.
Wu P, Wang P.Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17634-9. Epub 2004 Dec 13.
- 2.
- Comparison of enzymatic characterization and gene organization of cyclic nucleotide phosphodiesterase 8 family in humans.
Gamanuma M, Yuasa K, Sasaki T, Sakurai N, Kotera J, Omori K.Cell Signal. 2003 Jun;15(6):565-74.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of PDE8A polyclonal antibody (Cat # PAB1878) in HL-60 cell line lysates (35 ug/lane). PDE8A (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PDE8A polyclonal antibody (Cat # PAB1878) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 5151
- Protein Accession#:
- NP_002596;O60658
- Gene Name:
- PDE8A
- Gene Alias:
- FLJ16150,HsT19550
- Gene Description:
- phosphodiesterase 8A
- Omim ID:
- 602972
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP. These cyclic nucleotides play an important role as second messengers in multiple physiologic processes, including regulation of vascular resistance, cardiac output, visceral motility, immune response, inflammation, neuroplasticity, vision, and reproduction. PDEs comprise a large superfamily of enzymes divided into 10 families. Different PDEs can be distinguished by their structure, tissue expression, localization, substrate specificity, regulation, and sensitivity to PDE inhibitors. Diversity in structure and specificity of function make PDEs promising targets for the pharmacotherapy of diseases modulated by cyclic nucleotide signaling (Hetman et al., MIM 2000). See MIM 171885.[supplied by OMIM
- Other Designations:
- OTTHUMP00000192898,cAMP-specific cyclic nucleotide phosphodiesterase 8A,high-affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
- Gene Pathway