细胞名称：人结肠癌细胞（STR鉴定正确）

细胞别称：HCT-116; HCT.116; HCT_116; HCT116; CoCL2

HCT116是1979年BrattainM等从患结肠癌的男性病人中分离建立的3株恶性细胞之一；产生CEA、角蛋白。在半固体琼脂糖培养基中形成克隆。HCT116在无胸腺的裸鼠有致瘤性，形成上皮样的肿瘤。

细胞名称：人结肠癌细胞

细胞简称：HCT116

产品货号：TCH-C185

种属来源：人

组织来源：结肠

疾病特征：结肠癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系： McCoys 5A+10%FBS+1%P/S

传代比例：1:3-1:8，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

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