Disease:

Colorectal adenocarcinoma 

Origin:

 Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon 

Species:

Homo sapiens 

Tissue:

Colon 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

Tumorigenic, Modal no. 45 (2n=46) 

Patient:

Female, Caucasian, 58 yrs of age 

Medium:

MEM + 10% FBS 

Subculture:

1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:
 I 
Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Protein Expression:
 HLA A2, B13, B50, IL6, IL10, mucin 
Isoenzymes:
 ADA, 1; ES-D, 1; G6PD, B; PEP-D, 1; PGD, A; PGM1, 1; PGM3, 2 
DNA Profile (STR):
 Amelogenin: X ; CSF1PO: 10,13; D13S317: 10; D16S539: 11,13; D5S818: 10,15; D7S820: 11,9.3; THO1: 6,7; TPOX: 8,9; vWA: 15,18 
Mutations in LS-180 Cells 

Related cells by Organ:

Caco-2 cells 

DLD-1 cells 

HCA-7 cells 

HCT-116 cells 

SW480 cells 

HT-29 cells 

LS-180 cells 

SW620 cells 

T84 cells 

SW48 cells 

RKO cells 

SNU-407 cells 

SNU-503 cells 

LS-174T cells 

LoVo cells 

HCT15 cells 


References for LS-180 cells:

1. Bass, S. E., Sienkiewicz, P., Macdonald, C. J., Cheng, R. Y., Sparatore, A., Del Soldato, P., Roberts, D. D., Moody, T. W., Wink, D. A., and Yeh, G. C. Novel dithiolethione-modified nonsteroidal anti-inflammatory drugs in human hepatoma HepG2 and colon LS180 cells. Clin Cancer Res, 15: 1964-1972, 2009. 

2. Fukumori, S., Masago, M., Ishida, K., Kayano, Y., Taguchi, M., and Hashimoto, Y. Temperature-dependent specific transport of levofloxacin in human intestinal epithelial LS180 cells. Biopharm Drug Dispos, 30: 448-456, 2009. 

3. Graber-Maier, A., Buter, K. B., Aeschlimann, J., Bittel, C., Kreuter, M., Drewe, J., and Gutmann, H. Effects of Curcuma extracts and curcuminoids on expression of P-glycoprotein and cytochrome P450 3A4 in the intestinal cell culture model LS180. Planta Med, 76: 1866-1870. 

4. Ishida, K., Takaai, M., Yotsutani, A., Taguchi, M., and Hashimoto, Y. Membrane transport mechanisms of mizoribine in the rat intestine and human epithelial LS180 cells. Biol Pharm Bull, 32: 741-745, 2009. 

5. Takaai, M., Morishita, H., Ishida, K., Taguchi, M., and Hashimoto, Y. Contribution of Na+-independent nucleoside transport to ribavirin uptake in the rat intestine and human epithelial LS180 cells. Eur J Pharmacol, 601: 61-65, 2008. 

6. Yamasaki, D., Nakamura, T., Okamura, N., Kokudai, M., Inui, N., Takeuchi, K., Watanabe, H., Hirai, M., Okumura, K., and Sakaeda, T. Effects of acid and lactone forms of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors on the induction of MDR1 expression and function in LS180 cells. Eur J Pharm Sci, 37: 126-132, 2009.