MAP3K5 (Human) Recombinant Protein
产品名称： MAP3K5 (Human) Recombinant Protein
英文名称： MAP3K5 (Human) Recombinant Protein
产品编号： P4194
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- 邮编 : 11493
- 所在区域 : 台湾
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- Specification
- Product Description:
- Human MAP3K5 (Q99683, 649 a.a. - 946 a.a.) partial recombinant protein expressed in Sf9 cells.
- Theoretical MW (kDa):
- 60
- Form:
- Liquid
- Preparation Method:
- Insect cell (Sf9) expression system
- Purity:
- ≥ 95% by SDS-PAGE
- Activity:
- 97 nmol phosphate incorporated into MBP per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.25 mM Dithiothreitol, 0.1 mM EGTA, 0.1 mM EDTA, 25% glycerol, 0.1 mM PMSF)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- 1 ug/lane SDS-PAGE Stained with Coomassie Blue
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 4217
- GeneBank Accession#:
- NM_005923
- Protein Accession#:
- Q99683
- Gene Name:
- MAP3K5
- Gene Alias:
- ASK1,MAPKKK5,MEKK5
- Gene Description:
- mitogen-activated protein kinase kinase kinase 5
- Omim ID:
- 602448
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq
- Other Designations:
- MAP/ERK kinase kinase 5,MAPK/ERK kinase kinase 5,OTTHUMP00000017275,apoptosis signal regulating kinase
- Interactome
