• 偶联物Alexa Fluor® 647. Ex: 652nm, Em: 668nm
• 经测试应用ICC/IFmore details
• 种属反应性
  与反应: Rat
  预测可用于: Horse, Cow, Human, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Mouse NeuN aa 300 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
  (Peptide available as ab155068)

   

• 阳性对照
  • ICC/IF: PC12 cells
• 常规说明

  The Alexa Fluor^® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor^® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor^® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor^® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

  Alternative versions available:

  Anti-NeuN antibody - Neuronal Marker (ab128886)

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Stable for 12 months at -20°C. Store In the Dark.
• 存储溶液pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 30% Glycerol, 1% BSA
  
  Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
• 浓度100 µl 浓度为 0.5 mg/ml 
  
• 纯度Immunogen affinity purified
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Cell Type Marker
   
  • Neuron marker
   
  • Soma marker

  • Tags & Cell Markers
   
  • Cell Type Markers
   
  • Neuroscience Markers
   
  • Neuronal

  • Epigenetics and Nuclear Signaling
   
  • Transcription
   
  • Domain Families
  • Forkhead Box
   
  • Other FOXes

  • Epigenetics and Nuclear Signaling
   
  • Transcription
   
  • Transcription Factors

应用

靶标

• 功能RNA-binding protein that regulates alternative splicing events.
• 序列相似性Contains 1 RRM (RNA recognition motif) domain.
• 细胞定位Nucleus. Cytoplasm.

• Anti-NeuN antibody - Neuronal Marker (Alexa Fluor® 647) 图像

  • 

    Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody - Neuronal Marker (Alexa Fluor® 647) (ab196584)

    ab196584 staining NeuN in PC12 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196584 at 1/500 dilution (shown in red) andab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).