• 样品类型
  Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
• 检测类型Quantitative
• 检测时间
  6h 00m
• 种属反应性
  与反应: Cow
• 产品概述

  ab197410 can be used for quantitative measurement of 10 Bovine cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
  Targets names: IFNαA, IFNγ, IL-13, IL-1α, IL-1F5, IL-21, IP-10, MIG, MIP-1β, and TNFα.
  Learn more about quantitative antibody arrays

• 说明

  Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. 

  Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. 

  For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. 

  Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. 

  Learn more about quantitative antibody arrays 

  Learn more about membrane antibody arrays

性能

• 存放说明Store at -20°C. Please refer to protocols.

• 组件	8 tests	22 tests	50 tests
  20X Wash Buffer I	2 x 30ml	3 x 30ml	6 x 30ml
  20X Wash Buffer II	1 x 30ml	1 x 30ml	2 x 30ml
  Bovine Cytokine Array (10 T) Biotinylated Antibody Cocktail	1 unit	2 units	4 units
  Bovine Cytokine Array (10 T) Glass Slide	1 slide	2 slides	4 slides
  Bovine Cytokine Array (10 T) Lyophilized Cytokine Standard Mix	1 vial	2 vials	4 vials
  Cy3 equivalent dye-conjugated Streptavidin	1 x 5µl	2 x 5µl	4 x 5µl
  Sample Diluent	1 x 15ml	1 x 15ml	2 x 15ml
  Slide incubation chamber and frame	1 unit	2 units	4 units
  Slide Washer/Dryer (30 mL Centrifuge Tube)	1 unit	1 unit	2 units

• 研究领域
  • Immunology
   
  • Innate Immunity
   
  • Cytokines
   
  • Interleukins

  • Immunology
   
  • Innate Immunity
   
  • Cytokines
   
  • Interferons

  • Antibody Arrays
   
  • Membrane antibody arrays
   
  • Cytokine antibody arrays