重组人CD27蛋白（Recombinant Human CD27 Protein），GMP级

产品货号：GMP-TL709

产品描述（Description）

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD7 on lymphocytes and dendritic cells. The CD27-CD7 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.

分子别名（Synonym）

 CD27、TNFRSF7、S152、T14、Tp55

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列编码人CD27（NCBI Reference Sequence: NP_001233.1）表达带有Fc标签在C末端

分子量（Molecular Weight）

重组人 CD27 蛋白包含 394 个氨基酸，预测理论分子量为 46.2 kD

纯度（Purity）

≥95%采用SDS-PAGE凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人 CD27(FC 标签)蛋白的浓度 1μg/mL(100μL/孔)能够结合 CD27 Ligand(His 标签) 的线性范围是 0.8-15ng/mL

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、 CD27signalingonchronicmyelogenousleukemiastemcellsactivatesWnttargetgenesandpromotesdiseaseprogression.Christia nSchürch,CarstenRiether,MatthiasS.Matter,AlexandarTzankov,AdrianF.Ochsenbein.JClinInvest.2012Feb1;122(2):624–638.Pu blishedonline2012Jan9.doi:10.1172/JCI45977 

2、 CD27expressiondiscriminatesporcineThelpercellswithfunctionallydistinctproperties.KatharinaReutner,JudithLeitner,Andrea Müllebner,AndreaLadinig,SabineEEssler,JCatharinaDuvigneau,MathiasRitzmann,PeterSteinberger,ArminSaalmüller,Wilhelm GernerVetRes.2013;44(1):18.Publishedonline2013Mar11.doi:10.1186/1297-9716-44-18

  注意：For research and manufacturing use.