粪便基因组中量DNA提取试剂盒-核酸纯化-试剂-生物在线
北京天漠科技开发有限公司
粪便基因组中量DNA提取试剂盒

粪便基因组中量DNA提取试剂盒

商家询价

产品名称： 粪便基因组中量DNA提取试剂盒

英文名称： ZR Fecal DNA MidiPrep™

产品编号： D6110

产品价格： 0

产品产地： 美国

品牌商标： ZYMO RESEARCH

更新时间： 2023-09-19T17:25:32

使用范围： null

北京天漠科技开发有限公司
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  • 邮编 : 100101
  • 所在区域 : 北京
  • 电话 : +86-10-139****7531 点击查看
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ZR Fecal DNA MidiPrep™

 HIGHLIGHTS

•
Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in as little as 25 minutes including humans, birds, rats, mice, cattle, etc.
• State-of-the-art, ultra-high density BashingBeads™ are fracture resistant and chemically inert.
• Can be used with any bead mill, disrupter, or vortex that can accommodate standard 50 ml tubes.
• Omits the use of organic denaturants as well as proteinases.


SPECIFICATIONS
• Formats: Bead Beating, Spin/Vacuum Filtration, and Spin Column Purification
• Sample Sources: Bacterial, protist, as well as host DNA can be effectively isolated from a ≤ 375 mg sample of mammalian feces.
• DNA Purity: High quality, humic/fulvic-free DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260 nm/280 nm > 1.8, A260 nm/230 nm > 2.0
• DNA Size Limits: Capable of recovering genomic DNA sized fragments from 100 bp to ≥ 40 kb. Typical fragment sizes range from 25 kb-35 kb. If present, parasitic and viral DNA will also be recovered.
• DNA Recovery: Typically, up to ~125 µg total DNA is eluted into ≥150 µl DNA Elution Buffer per sample.
• Equipment: Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter.



DESCRIPTION

The ZR Fecal DNA MidiPrep™ is designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of sample sources including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in as little as 25 minutes: fecal samples (≤ 375 mg each) are added directly to a ZR BashingBead™ Lysis/Filtration Tube and rapidly and efficiently lysed by bead beating without the use of organic denaturants or proteinases. The DNA is then isolated and purified using our Fast-Spin column technology and is ideal for downstream molecular-based applications including PCR, arrays, genotyping, etc. The DNA is also well suited for methylation profiling purposes using any of our EZ DNA Methylation Kits. A schematic of the ZR Fecal DNA MidiPrep™ procedure is shown below.
 
The ZR Fecal DNA MidiPrep™ is designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of sample sources including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in as little as 25 minutes: fecal samples (d

Comparison of DNA yields from rat feces using the ZR Fecal DNA Kit™ and kits from competitors M and Q. Equivalent amounts of feces were processed using each kit and then equal volumes of eluted DNA were analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. Samples were processed in triplicate. Comparison of DNA yields from rat feces using the ZR Fecal DNA Kit™ and kits from competitors M and Q. Equivalent amounts of feces were processed using each kit and then equal volumes of eluted DNA were analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. Samples were processed in triplicate.
 
Panels A and B show the results of PCR with DNA isolated from rat and human fecal samples, respectively, using primers specific for prokaryotic 16S rRNA.
 
Panel C shows the results of PCR of DNA isolated from human feces with and without the addition of E. coli containing pUC19 plasmid DNA (indicated at the top of the image) using primers specific for the pUC19 sequence. In each case, amplicons were analyzed in a 1.5% (w/v) agarose/ethidium bromide gel using a UV imager. Numbers above each lane of the gel images are the volumetric equivalent (in µl) of eluted DNA (100 µl) used for PCR. Arrows mark the relative migration of amplicons in the gels, and M is a 100 bp DNA ladder (NEB).
 
PCR of DNAs from rat and human fecal samples isolated with the ZR Fecal DNA Kit™. Panels A and B show the results of PCR with DNA isolated from rat and human fecal samples, respectively, using primers specific for prokaryotic 16S rRNA. Panel C shows the results of PCR of DNA isolated from human feces with and without the addition of E. coli containing pUC19 plasmid DNA (indicated at the top of the image) using primers specific for the pUC19 sequence. In each case, amplicons were analyzed in a 1.5% (w/v) agarose/ethidium bromide gel using a UV imager. Numbers above each lane of the gel images are the volumetric equivalent (in µl) of eluted DNA (100 µl) used for PCR. Arrows mark the relative migration of amplicons in the gels, and M is a 100 bp DNA ladder (NEB).



ORDERING INFORMATION:
Products Description
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Cat No.
Size

Price

(RMB)

  

ZR Fecal DNA MidiPrep™

 Simple, rapid isolation of PCR-quality DNA from fecal samples in as little as 15 minutes.
D6110
25 preps.