Recombinant Human EGF Protein (rp145392)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9ng/mL.
Recombinant Human EGF Protein
产品名称： Recombinant Human EGF Protein
英文名称： Recombinant Human EGF Protein
产品编号： rp145392
产品价格： 0
产品产地： 上海
品牌商标： 阿拉丁
更新时间： 2023-08-04T14:27:25
使用范围： null
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基本信息
| 产品名称 | Recombinant Human EGF Protein |
|---|---|
| 英文名称 | Recombinant Human EGF Protein |
| 规格或纯度 | GMP, >95% SDS-PAGE, Active,Pichia pastoris, No tag, 971-1021aa |
| 生物活性 | Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL. |
| 内毒素水平 | < 0.2 EU/µg |
| 表达系统 | Pichia pastoris |
| 种属 | Human |
| 氨基酸 | 971-1021 aa |
| 序列 | NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWW ELR |
| 蛋白标签 | No tag |
| N-末端序列 | NSDSECPLSH |
| 蛋白长度 | Full length protein |
| Carrier Free | Yes |
| AnimalFree | Yes |
| Accession# | P01133 |
| 来源 | Recombinant |
| 预测分子量 | 6 kDa |
| SDS-PAGE | 6.6 kDa, under reducing conditions. |
相关属性
| 特点与好处 | ·Application:Cell Culture,FuncS,HPLC,MS,SDS-PAGE |
|---|---|
| 物理外观 | Lyophilized |
| 储存缓冲液 | 10mM磷酸钠缓冲液 |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 10mM PBS to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 在-20℃下稳定至 24 个月，在 2~8℃下稳定至 4 周。配制为 100μg/ml 的储存液，2~8℃可稳定保存一周。若需延长储存时间，建议在-70℃或-20℃冷冻，不建议重复冷冻和解冻。 |
常规信息
| 基因名 | EGF - epidermal growth factor |
|---|---|
| 基因 ID | 1950 |
| 细胞定位 | Membrane, Secreted |
| 序列相似性 | Contains 9 EGF-like domains. Contains 9 LDL-receptor class B repeats. |
| 涉及疾病 | Hypomagnesemia 4 |
| 基因别名 | HOMG4, URG |
| 英文别名 | beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone |
| 别名 | 重组人表皮生长因子蛋白(EGF)；beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone |
一般描述
Purity
≥ 95% SDS-PAGE. ≥ 95 % HPLC.
Additional sequence information
N-terminal glycine. Full-length mature EGF chain.
Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
Post-translational
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
图片
Recombinant Human EGF Protein (rp145392)-SDS-PAGE
5μg/lane of Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 6.6 kDa.