重组人GITR Ligand蛋白（Recombinant Human GITR Ligand Protein），GMP级

产品货号：GMP-TL721

产品描述（Description）

Tumor necrosis factor ligand superfamily member 18 (TNFSF18) is also known as Glucocorticoid - induced TNF-related ligand (GITRL or GITR Ligand), Activation - inducible TNF - related ligand (AITRL), which belongs to the tumor necrosis factor family. TNFSF18 is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. TNFSF18 / GITRL is up-regulated after stimulation by bacterial lipopolysaccharides (LPS). TNFSF18 Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. TNFSF18 / GITR Ligand is important for interactions between activated T-lymphocytes and endothelial cells. TNFSF18 also mediates activation of NF-kappa-B.

分子别名（Synonym）

TNFSF18、AITRL、TL6、hGITRL

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列编码人GITR Ligand（GenBank: AAD22634.1）表达带有Fc标签在C末端

分子量（Molecular Weight）

重组人GITR Ligand蛋白 包含359个氨基酸，预测理论分子量为41.2 kD

纯度（Purity）

≥95%采用SDS-PAGE凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人 GITR(His 标签)1ug/mL (100ul/孔)能够结合 GITR Ligand( Fc 标签) 的线性范围是 5-20ng/mL

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、Murine B Cell Development and Antibody Responses to Model Antigens Are Not Impaired in the Absence of the TNF Receptor GITR Lenka Sinik Teodorovic, Carlo Riccardi, Raul M. Torres, Roberta Pelanda PLoS One. 2012; 7(2): e31632. Published online 2012 Feb 6. doi: 10.1371/journal.pone.0031632 

2、 Pharmacological modulation of GITRL/GITR system: therapeutic perspectivesGiuseppe Nocentini, Simona Ronchetti, Maria Grazia Petrillo, Carlo Riccardi Br J Pharmacol. 2012 Apr; 165(7): 2089–2099. doi:10.1111/j.1476-5381.2011.01753.x 

3、 Agonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial gliomaMira A. Patel, Jennifer E. Kim, Debebe Theodros, Ada Tam, Esteban Velarde, Christina M. Kochel, Brian Francica, Thomas R. Nirschl, Ali Ghasemzadeh, Dimitrios Mathios, Sarah Harris-Bookman, Christopher C. Jackson, Christina Jackson, Xiaobu Ye, Phuoc T. Tran, Betty Tyler, Vladimir Coric, Mark Selby, Henry Brem, Charles G. Drake, Drew M. Pardoll, Michael Lim J Immunother Cancer. 2016; 4: 28. Published online 2016 May 17. doi: 10.1186/s40425-016-0132-2 Correction in: J Immunother Cancer. 2016; 4: 74

  注意：For research and manufacturing use.