SUPEROSE 6
产品名称： SUPEROSE 6
英文名称： SUPEROSE 6
产品编号： 17-5172-01
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
上海基星生物科技有限公司
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| 17-5057-01 | MICRORPC C2-C18 ST 4,6-100 |
| 17-5068-01 | SOURCE 15RPC ST 4.6/100 |
| 17-5116-01 | SOURCE 5RPC ST 4,6-150 |
| 17-5172-01 | SUPEROSE 6 10/300 GL |
| 17-5173-01 | SUPEROSE 12 10/300 GL |
| 17-5174-01 | SUPERDEX 75 10/300 GL |
| 17-5175-01 | SUPERDEX 200 10/300 GL |
| 17-5176-01 | SUPERDEX PEPTIDE 10/300 GL |
| 28-9065-61 | Superdex 200 5_150 GL |
| 28-9205-04 | Superdex 75 5_150 GL |
| 17-0506-01 | PP COLUMN MONO Q HR 16/10 |
| 17-0507-01 | PP COLUMN MONO S HR 16/10 |
Superose™ Columns and Lab Packs
- Achieve high-resolution separations across exceptionally broad molecular-weight ranges.
- Easy, efficient scale-up from prepacked Superose™ 10/300 GL columns to Superose™ prep grade lab packs.
- Low ionic strength eluents (< 0.05 M) can utilize a hydrophobic interaction component to alter the selectivity of Superose™ for some lipids, peptides, and small aromatic compounds.
- Tricorn™ high-performance column (10/300 GL) prepacked with Superose™ allows even distribution of eluent across the column bed to enable high-resolution separations.
- Precision columns (PC) for micropurification and analysis¾10-fold smaller column volume than Tricorn™ columns
Superose™ Columns and Lab Packs
Technical Information
Superose™ 6 is designed for high-resolution chromatography. Superose™ 6 prep grade permits easy, efficient scale-up to preparative separations. Superose™ 12 is for high resolution purification of proteins from 1 × 103 to 3 × 105 molecular weight, while Superose™ 12 prep grade is useful for preparative purification of these higher molecular weight proteins.
| TECHNICAL SPECIFICATIONS | |
| Superose™ 10/300 GL Columns (Tricorn™)* | |
| Bed volume | 24 ml |
| Sample loading capacity | 5-10 mg, 240 μl |
| Recommended flow rate | |
| Superose™ 6 | 0.3-0.5 ml/min |
| Superose™ 12 | 0.5-1.0 ml/min |
| Max. pressure | |
| Superose™ 6 | 15 bar (217 psi, 1.5 MPa) |
| Superose™ 12 | 30 bar (435 psi, 3 MPa) |
| Particle size | |
| Superose™ 6 | 13 ± 2 μm |
| Superose™ 12 | 10 ± 2 μm |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| Chemical stability | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place) |
| pH stability | 3-12 (working and long term) |
| 1-14 (short term) | |
| Theoretical plates | |
| Superose™ 6 | > 30 000 m-1 |
| Superose™ 12 | > 40 000 m-1 |
| Superose PC 3.2/30 | |
| Bed volume | 2.4 ml |
| Max. loading capacity | 200 μl |
| Max. flow rate | |
| Superose 6 | 0.1 ml/min |
| Superose 12 | 0.1 ml/min |
| Max. pressure | |
| Superose 6 | 12 Bar (1.2MPa / 175 psi) |
| Superose 12 | 24 Bar (2.4MPa / 350 psi) |
| Particle size | |
| Superose 6 | 13 ± 2 μm |
| Superose 12 | 10 ± 2 μm |
| Chemical stability | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place) |
| pH stability | 3-12 (working and long term) 1-14 (short term) |
| Theoretical plates | |
| Superose 6 | > 30 000 m-1 |
| Superose 12 | > 40 000 m-1 |
| * Columns are not suitable for use with ÄKTAprime™ plus system.
|
|
| TECHNICAL SPECIFICATIONS | |
| Superose™ prep grade | |
| Exclusion limit (Mr) | |
| Superose™ 6 prep grade | 4 × 107 protein |
| Superose™ 12 prep grade | 2 × 106 protein |
| Separation range (Mr) | |
| Superose™ 6 prep grade | 5 × 103-5 × 106 protein |
| Superose™ 12 prep grade | 1 × 103-3 × 105 protein |
| Matrix | Highly cross-linked agarose |
| Particle size | 30 ± 10 μm |
| Recommended linear flow rate | |
| Superose™ 6 prep grade | up to 40 cm/h |
| Superose™ 12 prep grade | up to 40 cm/h |
| Max. pressure | |
| Superose™ 6 prep grade: | 4 bar (58 psi, 0.4 MPa) |
| Superose™ 12 prep grade: | 7 bar (101 psi, 0.7 MPa) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| * Columns are not suitable for use with ÄKTAprime™ plus system.
|
|
. Numbers above peaks correspond to the number of base pairs. Reproduced by kind permission of the authors and publisher.'))
Separation of DNA fragments on Superose™ 6 HR 10/30 column (now available as Superose 6 10/300 GL column). Numbers above peaks correspond to the number of base pairs. Reproduced by kind permission of the authors and publisher.
References
- Andersson, T et al. Agarose-based media for high-resolution gel filtration of biopolymers. J. Chromatogr. 326, 33 (1985).
- Ellegren, H. and Låås, T. Size-exclusion chromatography of DNA restriction fragments. J. Chromatogr. 467, 217 (1989).
- Gao, Y. et al. A cytoplasmic chaperonin that catalyzes beta-actin folding. Cell 69, 1043 (1992).
- Lin, M. et al. Pyruvate kinase isoenzymes from Green Alga, Selenastrum minutum. Arch. Biochem. Biophys. 269, 219 (1989).
- Hei, Y. et al. Purification and characterisation of a novel ribosomal S6 kinase from skeletal muscle of insulin-treated rats. J. Biol. Chem. 269, 7816 (1994).
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