预优化DNA转染试剂-SiHa-克隆与表达-试剂-生物在线
济南思科生物科技有限公司
预优化DNA转染试剂-SiHa

预优化DNA转染试剂-SiHa

商家询价

产品名称： 预优化DNA转染试剂-SiHa

英文名称： GenJet™ In Vitro DNA Tranfection Reagent for SiHa Cells

产品编号： SL100489-SiHa

产品价格： 0

产品产地： 美国

品牌商标： SignaGen

更新时间： null

使用范围： null

济南思科生物科技有限公司
  • 联系人 :
  • 地址 : 济南市经六路689号绿地·玫瑰坊A座734号
  • 邮编 : 250021
  • 所在区域 : 山东
  • 电话 : 159****9896 点击查看
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  • 邮箱 : jnqsw@163.com; info@signagen-china.com
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Description

GenJet™ DNA In Vitro Tranfection Reagent for SiHa is pre-optimized for transfecting SiHa cells.  Refer to the following optimal transfection conditions for maximal transfection efficiency on SiHa cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency 

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)


~80%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours


EGFP
pEGFP-N3 (CMV promoter) 
76%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet.pdf