SPECIFICATION SHEET

Catalog #:                   L1C00201                    Lot #:C080301

Description:               MAb to Carcinoembryonic Ag

                                    Monoclonal Antibody to Carcinoembryonic Antigen (CEA)

Specificity:                  Human Carcinoembryonic Antigen

Clone:                         ML01C01

Host Animal:              Mouse.  Hybridization of X63-Ag8 myeloma cells with spleen cells from Balb/c mice.

Isotype:                      IgG₁

Source:                       Mouse Ascites

Immunogen:            CEA isolated from colorectal carcinomas by PCA extraction.

Format:                      Purified, Liquid

Purification:             Protein A chromatography followed by gel filtration.

Concentration:      2mg/ml (OD280nm, E^0.1% = 1.35)

Affinity Constant:    5.0 x 10^-9 mol/l

Buffer:                        0.01M  PH7.4  PBS

Preservative:             None

Applications:             Determination of CEA.  Each laboratory should determine an optimum working titer for use in its particular application.  Other applications have not been tested but use in such assays should not necessarily be excluded.

Recommended antibody pair for immunoassay:

                                    Capture                         Detection

                                    L1C00201                     L1C00202 

QC Information:      IgG >98% of total peak area (Superdex 200 gel chromatography on AktaFPLC)

Storage:                             Short-term (up to 2 months) store at < –20°C.  Long term (>2 months) store at <–40°C.