基本信息

产品名称Recombinant human FGF1 protein英文名称Recombinant human FGF1 protein规格或纯度Animal Free, >95% SDS-PAGE, Active, E.coli, No tag, 16-155 aa生物活性Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line in the presence of heparin. The ED₅₀ for this effect is typically <0.5ng/mL.内毒素水平< 0.1 EU/µg种属Human氨基酸Phe16-Asp155序列FNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAES VGEVYIKSTE TGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVG LKKNGSCKRG PRTHYGQKAILFLPLPVSSD蛋白标签No tag蛋白长度Full length proteinCarrier FreeYesAnimalFreeYesAccession#P05230来源Recombinant预测分子量15.9 kDaSDS-PAGE13.3 kDa, under reducing conditions; 13.3 kDa, under non-reducing conditions.

相关属性

特点与好处·Application:FuncS,SDS-PAGE,WB物理外观Lyophilized储存缓冲液Lyophilized from a sterile solution containing 50 mM Tris.Cl, pH7.5, 150mM NaCl.复溶After receiving lyophilized powder protein, centrifuge first, and then r econstitute at 100µg/mL in sterile PBS.储存温度-20°C储存运输条件超低温冰袋运输稳定性与储存Stable for 12 months from the date of receipt of the product under proper storage andhandling conditions. Avoid repeated freeze-thaw cycles.

常规信息

基因名FGF1基因 ID2246细胞定位Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SY序列相似性Belongs to the heparin-binding growth factors family.通路MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton基因别名AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1英文别名Acidic fibroblast growth factor aFGF Beta; endothelial cell growth factor Beta; endothelial cell growth factor; ECGF; ECGF beta; ECGF-beta; ECGFA; ECGFB; Endothelial Cell Growth Factor alpha; Endothelial Cell Growth Factor beta; FGF 1; FGF alpha; Fgf1; FG别名Acidic fibroblast growth factor aFGF Beta; endothelial cell growth factor Beta; endothelial cell growth factor; ECGF; ECGF beta; ECGF-beta; ECGFA; ECGFB; Endothelial Cell Growth Factor alpha; Endothelial Cell Growth Factor beta; FGF 1; FGF alpha; Fgf1; FG

一般描述

Purity

>95% as determined by SDS-PAGE and Coomassie blue staining

Function

The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.

Sequence similarities

Belongs to the heparin-binding growth factors family.

Cellular localization

Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.

图片

Protein Bioactivity: Recombinant Human FGF1 Protein (rp145907)

Recombinant Human FGF1 Protein (rp145907)-Protein Bioactivity

Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line in the presence of heparin. The ED50 for this effect is typically <0.5ng/mL.

SDS-PAGE: Recombinant Human FGF1 Protein (rp145907)

Recombinant Human FGF1 Protein (rp145907)-SDS-PAGE

3μg/lane of Recombinant Human FGF1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 13.3 kDa.