MSU诱导的腹膜炎模型
产品名称: MSU诱导的腹膜炎模型
英文名称: MSU induced peritonitis model
产品编号: BDA-IF007
产品价格: 询价
产品产地: null
品牌商标: null
更新时间: null
使用范围: null
上海百代安生物科技有限公司
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MSU诱导的腹膜炎模型
实验方案:
1) MSU用PBS重悬, 每只实验鼠腹腔注射1mgMSU;
2) 6小时后取腹腔灌洗液, 用10mL PBS冲洗;
3) 分析灌洗液中中性粒细胞数量,流式细胞检测,细胞表面分子: Ly-6G和CD11b
Brief protocol of MSU induced mouse peritonitis model:
1) Mice were i.p. injected of 1mg MSU crystals in 0.5 ml PBS;
2) 6h later, animals were killed by CO2 exposure;
3) Peritoneal cavities were washed with 10 ml of PBS;
4) The lavage fluids were analyzed for neutrophil recruitment by fluorescence-activated cell sorting using the neutrophil markers Ly-6G and CD11b