Colourimetric method for the determination of Amylose and 
Amylopectin in cereals, food and feed

Principle:
                                                                      (Con A)
(1) Soluble starch (amylose + amylopectin) → 
                                                        amylose + amylopectin-Con A
                                                        (soluble)         (precipitate)

                          (α-amylase + amyloglucosidase)
(2) Amylose (in solution) + H₂O → D-glucose

                                 (glucose oxidase)
(3) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

                                                                                    (peroxidase)
(4) H₂O₂ + p-hydroxybenzoic acid + 4-aminoantipyrine → 
                                                                       quinoneimine + H₂O
                                      
                                 (α-amylase + amyloglucosidase)
(5) Total starch (in solution) + H₂O → D-glucose

Kit size:                             100 assays
Method:                             Spectrophotometric at 510 nm
Total assay time:               ~ 120 min
Detection limit:                  Amylose 5-95% of total starch content
Application examples: 
Cereal starches, flours, pure starches and foods
Method recognition:         Novel method
Advantages
 
Very cost effective (cost per test) 
All reagents stable for > 12 months after preparation 
Only enzymatic kit available 
Accurate and reliable amylose / amylopectin ratio determination  
Simple format 
Standard included