该单克隆抗体是采用合成的与人源MEK1蛋白Ala220周围相一致的肽段免疫动物而产生的。此抗体通过胺基端和辣根过氧化物酶的碳水化合物端链接。此HRP偶联的抗体预期和未偶联的MEK1/2 (D1A5) Rabbit mAb #8727显示同样的物种交叉反应性。

Western Blotting

 

Western blot analysis of extracts from NIH/3T3 and HeLa cells using MEK1/2 (D1A5) Rabbit mAb (HRP Conjugate).

使用MEK1/2 (D1A5) Rabbit mAb兔单抗 (HRP Conjugate)对NIH/3T3和HeLa细胞提取物进行western blot分析。

 

Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

MEK1、MEK2,也被称为MAPK或Erk激酶，它们是双特异性蛋白激酶，在丝裂原活化蛋白激酶级联反应中发挥作用，控制细胞生长和分化(1-3)。MEK1、MEK2的活化是通过Raf样分子对两个丝氨酸（217 /221位点）的磷酸化而实现的，这两个丝氨酸位点位于亚结构域VIII的活化环内。多种生长因子和细胞因子以及膜去极化和钙内流都能激活MEK1/2(1-4)。组成型活化的MEK1/2能够诱导NIH/3T3细胞的转化和PC-12细胞的分化(4)。MEK蛋白通过将p44、p42 MAPK激酶上位于亚结构域VIII的活化环内的丝氨酸和苏氨酸磷酸化，激活p44、 p42 MAPK激酶。