重组人Galectin-9蛋白（Recombinant Human Galectin-9 Protein），GMP级

产品货号：GMP-TL719

产品描述（Description）

Galectin-9 (LGALS9) is also known as tumor antigen HOM-HD-21 and ecalectin. Galectins are a family of proteins defined by their binding specificity for β-galactoside sugars, such as N-acetyllactosamine (Galβ1-3GlcNAc or Galβ1-4GlcNAc), which can be bound to proteins by either N-linked or O-linked glycosylation. As for Galectin-9, it has high affinity for the Forssman pentasaccharide, and it is also the ligand for HAVCR2/TIM3. Forthermore, Galectin-9 stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth.

分子别名（Synonym）

LGALS9、Ecalectin

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列编码人Galectin-9（NCBI ReferenceSequence:NP_002299.2）表达带有His标签在C末端

分子量（Molecular Weight）

重组人Galectin-9蛋白 包含323个氨基酸，预测理论分子量为37.4 kD

纯度（Purity）

≥95%采用 SDS-PAGE 凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定蛋白检测其对 Jurkat 细胞（人急性 T 淋巴白血病细胞）的黏附迁移性的作用，该作用的 ED₅₀是 1.1-4.2ug/mL

稳定性（Stability）

冻干制剂可在 4℃保存 24 个月， 溶解后的液体可置于-20℃保存 6-12 个月， 避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、Astrocyte galectin-9 potentiates microglial TNF secretionAndrew J Steelman, Jianrong Li J Neuroinflammation. 2014; 11: 144. Published online 2014 Aug 27. doi: 10.1186/s12974-014-0144-0 

2、Galectin-9 binds IgM-BCR to regulate B cell signaling Anh Cao, Nouf Alluqmani, Fatima Hifza Mohammed Buhari, Laabiah Wasim, Logan K. Smith, Andrew T. Quaile, Michael Shannon, Zaki Hakim, Hossai Furmli, Dylan M. Owen, Alexei Savchenko, Bebhinn Treanor Nat Commun. 2018; 9: 3288. Published online 2018 Aug 17. doi: 10.1038/s41467-018-05771-8 

3、Cancer Therapy Due to Apoptosis: Galectin-9 Koji Fujita, Hisakazu Iwama, Kyoko Oura, Tomoko Tadokoro, Eri Samukawa, Teppei Sakamoto, Takako Nomura, Joji Tani, Hirohito Yoneyama, Asahiro Morishita, Takashi Himoto, Mitsuomi Hirashima, Tsutomu Masaki Int J Mol Sci. 2017 Jan; 18(1): 74. Published online 2017 Jan 1. doi: 10.3390/ijms18010074

  注意：For research and manufacturing use.