The ZR DNA Sequencing Clean-up Kit™ provides a simple method for the rapid removal of post-cycle sequencing reaction contaminants (i.e., unincorporated fluorescent dyes, residual salts, dNTPs, primers, and enzymes) from DNA extension products. These contaminants can often interfere with the quality and signal strength of sequencing data. In particular, unincorporated dyes can result in dye peaks (“dye blobs”) which may obscure portions of the sequencing chromatogram and interfere with base-calling accuracy of sequencing analysis software.

The ZR DNA Sequencing Clean-up Kit™ employs a single-buffer system that allows for efficient DNA adsorption onto the matrix of the supplied Zymo-Spin™ IB Column. The DNA is washed then eluted with a small volume of water or loading dye containing formamide. The entire DNA purification procedure typically takes about 2 minutes.