GSTRAP HP-色谱柱 -耗材-生物在线
上海基星生物科技有限公司
GSTRAP HP

GSTRAP HP

商家询价

产品名称： GSTRAP HP

英文名称： GSTRAP HP

产品编号： 17-5281-01

产品价格： 0

产品产地： 美国

品牌商标： GE

更新时间： null

使用范围： null

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17-5281-01 GSTRAP HP 5 X 1 ML
17-5281-05 GSTRAP HP 100 X 1 ML
17-5282-01 GSTRAP HP 1 X 5 ML
17-5282-02 GSTRAP HP 5 X 5 ML
17-5282-05 GSTRAP HP, 100 X 5 ML
17-5286-01 HISTRAP FF CRUDE 5X5 ML
17-5286-02 HISTRAP FF CRUDE 100X5 ML
17-5319-01 HISTRAP FF 5 X 1 ML
17-5319-02 HISTRAP FF 100 X 1 ML
17-5441-22 HITRAP CAPTO S, 5X1 ML
17-5441-23 HITRAP CAPTO S, 5X5 ML
17-5468-51 HiTrap Capto SP ImpRes 5x1mL
17-5468-55 HiTrap Capto SP ImpRes 5x5mL
17-5470-51 HiTrap Capto Q ImpRes 5x1mL
17-5470-55 HiTrap Capto Q ImpRes 5x5mL
17-6002-33 HITRAP IEX SELECTION KIT
18-1142-75 REC PROTEIN PURIF HANDBOOK
28-4010-81 HITRAP_HIPREP 1_16 CONNEC AKTA
28-4014-77 HISTRAP FF CRUDE KIT
28-4017-45 GSTRAP 4B, 5 X 1 ML
28-4017-46 GSTRAP 4B, 100 X 1 ML
28-4017-47 GSTRAP 4B, 1 X 5 ML
28-4017-48 GSTRAP 4B, 5 X 5 ML
28-4017-49 GSTRAP 4B, 100 X 5 ML
28-4058-44 HiTrap Capto Adhere 5x1ml
28-4058-46 5x5 ml Hitrap Capto adhere
28-4082-53 HITRAP MABSELECT, 5 X 1 ML
28-4082-55 HITRAP MABSELECT, 1 X 5 ML
28-4082-56 HITRAP MABSELECT, 5 X 5 ML
28-4082-58 HITRAP MABSELECT XTRA, 5X1ML
28-4082-60 HITRAP MABSELECT XTRA, 1X5ML
28-4082-61 HITRAP MABSELECT XTRA, 5X5ML
28-4110-01 HiTrap Butyl HP, 5x1ml
28-4110-05 HiTrap Butyl HP, 5 x 5ml
28-4110-07 HiTrap HIC Selection Kit, 7x1
28-9075-46 StrepTrap HP 5 x 1mL
28-9075-47 StrepTrap HP 1 x 5mL
28-9075-48 StrepTrap HP 5 x 5mL
28-9165-37 HiTrap Capto DEAE, 5X1ML
28-9165-40 HiTrap Capto DEAE, 5X5ML
28-9187-78 MBPTrap 5 x 1mL
28-9187-79 MBPTrap 1 x 5 mL
28-9187-80 MBPTrap 5 x 5mL
28-9343-88 HiTrap Capto IEX Selection Kit
28-9520-85 HiTrap Con A 4B 5 x 1 ml
28-9520-96 HiTrap Con A 4B 5 x 5 ml
 

GSTrap™ HP Columns - Easy, High-performance Purification

  • Excellent for high-performance purification of GST-tagged* proteins.
  • Fast, one-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins.
  • Glutathione coupled to Sepharose High Performance.
  • Simple operation of GSTrap HP using a syringe, a pump or a chromatography system, such as ÄKTAdesign.

GSTrap™ HP Columns - Easy, High-performance Purification

Technical Information


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GSTrap™ HP 1-ml and 5-ml columns are designed for high-performance purification of GST-tagged proteins.

 

GSTrap™ HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose™ High Performance medium, which provides high-resolution purifications, a more concentrated sample, and high binding capacity.

 

GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose™ High Performance. GST-tagged proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids, see pGEX Vectors (GST Gene Fusion System). GST-tagged proteins can be detected using colorimetric or immunological methods.

 

For more information of the full range of products included in the Glutathione S-transferase (GST) Gene Fusion System, see Introduction to Glutathione S-Transferase (GST) Gene Fusion System. A more comprehensive guide to the GST Gene Fusion System is available in handbook 18-1157-58, see Media Reference Books, Software and Video Cassettes.

 

TECHNICAL SPECIFICATIONS
Medium  Glutathione Sepharose™ High Performance 
Binding capacity*  » 10 mg recombinant GST/ml medium 
Column volume  1 ml and 5 ml 
Column dimensions  0.7 × 2.5 cm (1 ml); 1.6 × 2.5 cm (5 ml) 
Recommended flow rate†  0.2-2 ml/min (1 ml); 1-10 ml/min (5 ml) 
Max. pressure†  3 bar (0.3 MPa, 42 psi) 
pH stability  3-12 
Storage  20% ethanol 
Storage temperature  4°C to 30°C 
* Binding capacity will vary depending of the type of cell lysate, target protein, flow rate, temperature, pH, etc. This is an important consideration, especially during sample loading and elution.
† H2O at room temperature.

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