Zymoprep™ is the new, simple and efficient yeast plasmid miniprep that is based on the old E. coli alkaline lysis method with our Zymolyase added in the first solution. There is no need for glass beads, phenol, or vortexing. Reliably recover your plasmid from yeast cells every time whether you use colonies, patches on plates, or liquid cultures. The total plasmid yield is 0.01-0.3 ng for most 2µ based plasmids from 1.5 ml overnight cultures. This kit also works well with low-copy number yeast plasmids. The recovered plasmid is in TE buffer and can be used for E. coli transformations, Western blots, PCR, etc.