植物/种子基因组中量DNA提取试剂盒-核酸纯化-试剂-生物在线
北京天漠科技开发有限公司
植物/种子基因组中量DNA提取试剂盒

植物/种子基因组中量DNA提取试剂盒

商家询价

产品名称： 植物/种子基因组中量DNA提取试剂盒

英文名称： ZR Plant/Seed DNA MidiPrep™

产品编号： D6120

产品价格： 0

产品产地： 美国

品牌商标： ZYMO RESEARCH

更新时间： null

使用范围： null

北京天漠科技开发有限公司
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  • 地址 : 北京市朝阳区北苑路170号凯旋城E座1809室
  • 邮编 : 100101
  • 所在区域 : 北京
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ZR Plant/Seed DNA MidiPrep™

 HIGHLIGHTS

•
Simple method for the isolation of inhibitor-free, PCR-quality DNA from a variety of plant and seed samples in as little as 25 minutes.
• State-of-the-art, ultra-high density BashingBeads™ are fracture resistant and chemically inert.
• Can be used with any bead mill, disrupter, or vortex that can accommodate standard 50 ml conical centrifuge tubes.
• Omits the use of organic denaturants as well as proteinases.


SPECIFICATIONS
• Formats: Bead Beating, Spin/Vacuum Filtration, and Spin Column Purification.
• Sample Sources: Up to 375 mg that include leaves, stems, buds, flowers, fruit, seeds, etc.
• DNA Yield: Typically 100-400 ng DNA/mg plant material.
• DNA Purity: High quality, humic/fulvic-free DNA is eluted with DNA Elution Buffer
making it perfect for PCR. A260 nm/280 nm > 1.8, A260 nm/230 nm > 2.0
• DNA Size Limits: Capable of recovering genomic DNA sized fragments from 100 bp to ≥ 40 kb. Typical fragment sizes range from 25 kb-35 kb. If present, parasitic and viral DNA will also be recovered.
• DNA Recovery: Typically, up to~ 125 µg total DNA is eluted into ≥ 150 µl DNA Elution Buffer per sample.
• Equipment: Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter.



DESCRIPTION

The ZR Plant/Seed DNA MidiPrep™ is designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in as little as 25 minutes: plant samples (≤ 375 mg each) are added directly to a ZR BashingBead™ Lysis/Filtration Tube and rapidly and efficiently lysed by bead beating without the use of organic denaturants or proteinases. Polysaccharides and polyphenols/tannins are removed from the DNA using our Fast-Spin column and Zymo-Spin™ IV-HRC Column technologies, respectively. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc. A schematic of the ZR Plant/Seed DNA MidiPrep™ procedure is shown below.
The ZR Plant/Seed DNA MidiPrep™ is designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in as little as 25 minutes: plant samples (d

 
Figure 1. Comparison of DNA yields from various plant and seed samples using the ZR Plant/Seed DNA Kit™. Equivalent amounts of plant materials were processed with equal volumes of eluted DNA analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 1 kb DNA size marker (Zymo Research Corp.). 1 Arabidopsis thaliana 2 juniper 3 milkweed leaf 4 milkweed leaflet 5 milkweed preflowering bud 6,7 corn kernel 8,9 sunflower seed.
Figure 1. Comparison of DNA yields from various plant and seed samples using the ZR Plant/Seed DNA Kit™. Equivalent amounts of plant materials were processed with equal volumes of eluted DNA analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 1 kb DNA size marker (Zymo Research).
Figure 2. PCR of diluted DNA (0 to 0.001) isolated with the ZR Plant/Seed DNA Kit™ from Arabidopsis thaliana leaf samples demonstrates the effectiveness of the Zymo-Spin™ IV-HRC Column at removing PCR inhibitors from the DNA. The arrow shows the relative migration of a ~700 bp amplicon from Chromosome 1 in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 100 bp DNA size marker (Zymo Research Corp.)
Figure 2. PCR of diluted DNA (0 to 0.001) isolated with the ZR Plant/Seed DNA Kit™ from Arabidopsis thaliana leaf samples demonstrates the effectiveness of the Zymo- Spin™ IV-HRC Column at removing PCR inhibitors from the DNA. The arrow shows the relative migration of a ~700 bp amplicon from Chromosome 1 in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 100 bp DNA size marker (Zymo Research Corp.)

 
Figure 3. PCR of diluted DNA (0 to 0.001) isolated with the ZR Plant/Seed DNA Kit™ from Arabidopsis thaliana leaf samples demonstrates the effectiveness of the Zymo-Spin™ IV-HRC Column at removing PCR inhibitors from the DNA. The arrow shows the relative migration of a ~700 bp amplicon from Chromosome 1 in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 100 bp DNA size marker (Zymo Research Corp.)
Figure 3. PCR of diluted DNA (0 to 0.001) isolated with the ZR Plant/Seed DNA Kit™ from corn kernels. The arrow shows the relative migration of a ~450 bp amplicon from mitochondrial DNA in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 100 bp DNA size marker (Zymo Research Corp.)



ORDERING INFORMATION:
Products Description
Protocol
Cat No.
Size

Price

(RMB)

  

ZR Plant/Seed DNA MidiPrep™

 Simple, rapid isolation of inhibitor-free, PCR-quality DNA from plant and seed samples.
D6120
25 preps.