Anti-14-3-3 theta抗体-抗体-抗体-生物在线
深圳市宇德立生物科技有限公司
Anti-14-3-3 theta抗体

Anti-14-3-3 theta抗体

商家询价

产品名称： Anti-14-3-3 theta抗体

英文名称： 14-3-3 antibody 14-3-3 protein T-cell antibody 14-3-3 protein tau antibody 14-3-3 protein theta antibody 1C5 antibody HS1 antibody theta polypeptide a

产品编号： ab183075

产品价格： null

产品产地： 英国

品牌商标： abcam

更新时间： 2023-09-21T08:08

使用范围：

深圳市宇德立生物科技有限公司
  • 联系人 :
  • 地址 : 深圳市宝安区西乡宝民二路贤基大厦4E
  • 邮编 :
  • 所在区域 : 广东
  • 电话 : 133****4454 点击查看
  • 传真 : 点击查看
  • 邮箱 : 1484332550@qq.com
进入展台

 

  • 特异性ab183075 detects endogenous level of total 14-3-3 theta protein.
  • 经测试应用WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Orangutan
  • 免疫原

    Synthetic peptide within Human 14-3-3 theta aa 140-154. The exact sequence is proprietary. Genbank: NP_006817
    Sequence:

    QTIDNSQGAYQEAFD


    Database link: P27348

     

    Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Mouse brain, Human lymphoma and Human colon cancer tissue lysates; Human A549 and mouse NIH 3T3 cell lysates; Human tonsil tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存���溶液pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 49% PBS

    PBS is without Mg2+ and Ca2+
  • 浓度100 µl 浓度为 0.6 mg/ml 
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab183075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Abreviews 说明
WB   1/300 - 1/2000. Predicted molecular weight: 28 kDa.
IHC-P   1/20 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-14-3-3 theta antibody 图像

  • Western blot - Anti-14-3-3 theta antibody (ab183075)
    Western blot - Anti-14-3-3 theta antibody (ab183075)
    All lanes : Anti-14-3-3 theta antibody (ab183075) at 1/300 dilution

    Lane 1 : mouse brain tissue lysate
    Lane 2 : Human A549 cell lysate
    Lane 3 : Human lymphoma tissue lysate
    Lane 4 : mouse NIH 3T3 cell lysate
    Lane 5 : Human colon cancer tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
    developed using the ECL technique

    Predicted band size : 28 kDa


    Exposure time : 5 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 theta antibody (ab183075)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 theta antibody (ab183075)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 14-3-3 theta with ab183075 at a 1/20 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)