• 特异性This antibody detects endogenous level of total 14-3-3 epsilon protein.
• 经测试应用ICC/IF, IHC-P, WBmore details
• 种属反应性
  与反应: Mouse, Rat, Human, African Green Monkey
• 免疫原

  Recombinant full length protein corresponding to Human 14-3-3 epsilon aa 1-255.
  Sequence:

  MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYK NVIGARRASW RIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICC DILDVLDKHLIPAANTGESKVF YYKMKGDYHRYLAEFATGNDRKEAAE NSLVAYKAASDIAMTELPPTHPIRLGLALNFSVF YYEILNSPDRACRL AKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGE EQ NKEALQDVEDENQ

  
  Database link: P62258

   

  Run BLAST with Run BLAST with 
• 阳性对照
  • T47D, Jurkat, PC12, COS1, COS7, NIH3T3 and SW480 cell extracts.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液pH: 7.4
  Preservative: 0.02% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
  
  without Mg2+ and Ca2+
• 浓度100 µl 浓度为 1 mg/ml 
  
• 纯度Immunogen affinity purified
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Neurology process
   
  • Neural Signal Transduction

  • Signal Transduction
   
  • Adapters
   
  • Cytoplasmic

  • Neuroscience
   
  • Cell Type Marker
   
  • Neuron marker
   
  • Synapse marker

  • Stem Cells
   
  • Signaling Pathways
   
  • Wnt
   
  • 14-3-3

应用

靶标

• 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
• 序列相似性Belongs to the 14-3-3 family.
• 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
• Information by UniProt
• 数据库链接
  • Entrez Gene: 7531 Human
  • Entrez Gene: 22627 Mouse
  • Entrez Gene: 29753 Rat
  • Omim: 605066 Human
  • SwissProt: P62258 Human
  • SwissProt: P62259 Mouse
  • SwissProt: P62260 Rat
  • Unigene: 513851 Human

  see all
• 别名
  • 14 3 3 E antibody
  • 14 3 3 epsilon antibody
  • 14 3 3E antibody

  see all

Anti-14-3-3 epsilon antibody 图像

• 

  Western blot - Anti-14-3-3 epsilon antibody (ab196891)
  All lanes : Anti-14-3-3 epsilon antibody (ab196891) at 1/500 dilution
  
  Lane 1 : T47D cell extract
  Lane 2 : Jurkat cell extract
  Lane 3 : PC12 cell extract
  Lane 4 : COS1 cell extract
  Lane 5 : COS7 cell extract
  Lane 6 : NIH 3T3 cell extract
  Lane 7 : SW480 cell extract
  
  
  Predicted band size : 29 kDa