重组人TIM-3蛋白（Recombinant Human TIM-3 protein），GMP级

产品货号：GMP-TL731

产品描述（Description）

Hepatitis A virus cellular receptor 2 is also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

分子别名（Synonym）

HAVCR2、TIM3、TIMD3、FLJ14428、KIM3

表达宿主（Expression host）

HEK293 细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA 序列编码人 TIM3（ NCBI Reference Sequence:NP_116171.3）表达带有 Fc 标签在 C 末端

分子量（Molecular Weight）

重组人 TIM-3 蛋白包含 412 个氨基酸，预测理论分子量为 49.5 kD

纯度（Purity）

≥95%采用 SDS-PAGE 凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性 （Biological Activity）

固定人 TIM-3(Fc 标签)1ug/mL (100uL/孔) 能够结合抗人 TIM-3 的抗体的线性范围是 0.1-5ng/mL

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

参考文献

1、 Functional variants of TIM-3/HAVCR2 3′UTR in lymphoblastoid cell lines Feifei Pu, Fengxia Chen, Zhicai Zhang, Jing Feng, Ping Xia Future Sci OA. 2018 Jun; 4(5): FSO298. Published online 2018 Mar 15. doi: 10.4155/fsoa-2017-0121 

2、 Unique Immune Gene Expression Patterns in Bronchoalveolar Lavage and Tumor Adjacent Non-Neoplastic Lung Tissue in Non-Small Cell Lung CancerChih-Hsi Scott Kuo, Chien-Ying Liu, Stelios Pavlidis, Yu-Lun Lo, Yen-Wen Wang, Chih-Hung Chen, How-Wen Ko, Fu-Tsai Chung, Tin-Yu Lin, Tsai-Yu Wang, Kang-Yun Lee, Yi-Ke Guo, Tzu-Hao Wang, Cheng-Ta Yang Front Immunol. 2018; 9: 232. Published online 2018 Feb 12. doi: 10.3389/fimmu.2018.00232

  注意：For research and manufacturing use.