大鼠心肌梗塞动物模型实验方案

冠状动脉左前降支结扎

（1）实验开始前大鼠随机分组；
（2）麻醉大鼠后，大鼠开胸后结扎冠状动脉左前降支；
（3）结扎成功的大鼠可见灰白的缺血区，心电图显示T波抬高；

梗塞面积测量

（4）实验结束时取心脏，切成薄片；
（5）TTC染色后甲醛固定；
（6）根据染色结果计算梗塞面积。

Breif protocol of myocardial infaction (MI )
model

Left coronary artery
occlusion

(1)Studies were performed on rats. Animals were randomly divided
into several groups before study.
(2)Each rat was anesthetized, and a left
thoracotomy was performed to exteriorize the heart rapidly by gentle pressure on
the right side of the thorax.
(3)The left anterior descending coronary
artery was permanently ligated using 7-0 silk suture 2-3 mm from its origin
between the pulmonary artery conus and the left atrium.
(4)Successful
ligation of the coronary artery was evidenced by regional cyanosis immediately
in the ischemic area (anterior ventricular wall and the apex) of the heart with
color change greater than 40% of left ventricular and confirmed by
ECG.
(5)The heart was then returned to its normal position and the thorax
immediately closed.
(6)Surviving rats were maintained on standard rat chow
and water ad libitum.


Infarct Size
Measurement


(7)The entire ventricular tissue was dissected, cut into six
horizontal slices and sections.
(8)After incubation in 2,3,5-triphenyl
tetrazolium chloride (TTC) solution. After that the sections were immersed in
formalin.
(9)After fixing, slices were photographed and captured separately
as digital images. Total area and infarct area were determined by computerized
planimetry. Percentage infarct size was calculated as the infarct area/total
left ventricular area.



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