The KAPA LTP Library Preparation Kit is designed for the
construction of libraries for Illumina® sequencing, starting
from fragmented, double-stranded DNA. The kit provides
all of the enzymes and reaction buffers required for the
following steps of library construction:

1. End repair, which produces blunt-ended, 5-phosphorylated
fragments.
2. A-tailing, during which dAMP is added to the 3-ends
of blunt-ended dsDNA library fragments.
3. Adapter ligation, during which dsDNA adapters with
3-dTMP overhangs are ligated to 3-A-tailed library
fragments.
4. Library amplification (optional), which employs PCR
to amplify library fragments carrying appropriate
adapter sequences on both ends.

Product Applications
The KAPA LTP Library Preparation Kit is ideally suited
for low-throughput, manual NGS library construction
workflows that involve end repair, A-tailing, adapter
ligation, and library amplification (optional). The protocol
may be adapted for incorporation into workflows for a
wide range of NGS applications, including:
• Whole-genome shotgun sequencing
• Targeted sequencing by solution hybrid selection
(i.e. exome or custom capture using the Roche
Nimblegen™, Agilent SureSelect, Illumina® TruSeq™, or
IDT xGen™ Lockdown™ Probes systems)
• ChIP-seq
• RNA-seq
• Methyl-seq (in combination with the KAPA HiFi
HotStart Uracil+ ReadyMix)
Specific guidelines for the construction of libraries for
target enrichment using the Roche Nimblegen™ SeqCap
EZ system may be found in Appendix 1.

KAPA LTP Library Preparation Kit 操作说明书.pdf